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The ring-shaped cohesin complex links sister chromatids until their timely segregation during mitosis. Cohesin is enriched at centromeres, where it provides the cohesive counter-force to bi-polar tension produced by the mitotic spindle. As a consequence of spindle tension, centromeric sequences tran...
ORGANISM(S): Saccharomyces cerevisiae 
Type of experiment: ChIP-chip (Chromatin immunoprecipitation on DNA chip) analyses of replication related, checkpoint related proteins and BrdU incorporated regions at 300bp resolution. Newly developed S.cerevisiae chromosome VI tiling array produced by affymetrix was used. Experimental factors:Dis...
ORGANISM(S): Saccharomyces cerevisiae 
RD3-KO Y-79 cells and RD3-SPOT-expressing cells were fixed by adding 27 µl of 37% formaldehyde (Wako) (final: 0.1%), incubated at RT for 10 min, quenched with 1 ml of 1 M Glycine-NaOH pH 7.5 (Nacalai) (final: 100 mM) at RT for 4 min, washed with 10 ml DPBS, and stocked at -80℃ as a cell pellet. For ...
ORGANISM(S): Homo Sapiens (human) 
Tumor and non-tumor tissues from 19 cases of colorectal cancer were examined using DIA-based mass spectrometry. A total of 11,833 proteins were identified, including 531 of the 748 cancer-associated proteins defined in the Catalogue of Somatic Mutations in Cancer. In the comparative analysis between...
ORGANISM(S): Homo Sapiens (human) 
Cell lysates were prepared from Tmem63b-GFP-expressing BDKO cells and incubated with the GFP-Trap magnetic agarose beads (Proteintech). After rotation at 4˚C for 3 hr, the beads were precipitated with the magnet, followed by washing several times using wash buffer containing 25 mM Tris-HCl (pH 8.0),...
ORGANISM(S): Mus Musculus (mouse) 
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