In coronavirus (CoV) infection, polyproteins (pp1a/pp1ab) are processed into non-structural proteins (nsps), which largely form the replication/transcription complex (RTC). The polyprotein processing and complex formation is critical and offers potential therapeutic targets. However, the interplay o...
Human noroviruses (HuNV) are members of the Caliciviridae family and are a significant cause of gastroenteritis worldwide. Cell culture models are challenging for studying the intracellular replication of HuNV, with murine norovirus (MNV) routinely used to study norovirus replication. Norovirus geno...
Creation of dual EMCV-IRES-integrated dengue virus translation suppression of non-structural polyprotein and induction of cellular Mdm2 oncoprotein suppress the viral replication in mosquito cells and mammalian cells, respectively.
Ribosome profiling of enterovirus infected cells was used to investigate expression of the virus polyprotein and upstream open reading frames. Samples were either pretreated with initiation inhibitor lactimidomycin (LTM) to cause ribosomes to accumulate at initiation sites, or were flash frozen with...
