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ProteomicsQuantitative proteomic analysis of trypsin-treated extracellular vesicles to evaluate non-vesicular contaminants and surface vesicular proteins.
Extracellular vesicles (EVs) are lipid bilayered vesicles released into extracellular milieu by most of cells. Currently, various purification methods of EVs have been established but most of studies use ultracentrifugation-based purification of EVs. However, these methods have the problem of co-pur...
ORGANISM(S): Homo sapiens (Human) 
2021-08-30 | PXD001355 | Pride
Non-vesicular contaminants Quantitative proteomics Extracellular vesicles Trypsin treatment 
ProteomicsA High-Resolution Proteomic Atlas of Extracellular Vesicles and Non-Vesicular Particles
Extracellular vesicles (EVs) are key mediators of intercellular communication and promising biomarkers. However, their molecular characterization remains challenging due to the heterogeneity of EV subtypes and co-isolated nanoparticles. Here, leveraging protein correlation profiling along density gr...
ORGANISM(S): Homo sapiens (Human) 
2025-12-30 | PXD062477 | Pride
Density gradient Proteomics Extra-cellular vesicles Biofluids Cancer cells 
ProteomicsA High-Resolution Proteomic Atlas of Extracellular Vesicles and Non-Vesicular Particles
Extracellular vesicles (EVs) are key mediators of intercellular communication and promising biomarkers. However, their molecular characterization remains challenging due to the heterogeneity of EV subtypes and co-isolated nanoparticles. Here, leveraging protein correlation profiling along density gr...
ORGANISM(S): Homo sapiens (Human) 
2025-12-30 | PXD062547 | Pride
Density gradient Proteomics Extra-cellular vesicles Biofluids Cancer cells 
UnknownExtracellular small non-coding RNA contaminants in fetal bovine serum and serum-free media.
Not available
S-EPMC6445286 | biostudies-literature
UnknownSystematic characterization of mammalian extracellular vesicles using nano-flow cytometry.
Not available
S-EPMC12707723 | biostudies-literature
UnknownQuality and efficiency assessment of six extracellular vesicle isolation methods by nano-flow cytometry.
Not available
S-EPMC6896440 | biostudies-literature
ProteomicsLC-MS of FAPS-sorted ELVAs from mouse oocytes
We discovered that mouse oocytes contain novel non-membrane-bound organelles which we named EndoLysosomal Vesicular Assemblies (ELVAs). ELVAs contain endolysosomal and autophagy vesicles as well as Proteasomes held together by a matrix protein. To discover the matrix protein holding ELVAs together w...
ORGANISM(S): Mus musculus (Mouse) 
2024-02-20 | PXD042768 | Pride
Mouse Oocyte Vesicular assemblies Faps 
ProteomicsMass Spectrometry of FAPS sorted ELVAs by an alternative sorting strategy
We discovered that mouse oocytes contain novel non-membrane-bound organelles which we named EndoLysosomal Vesicular Assemblies (ELVAs). ELVAs contain endolysosomal and autophagy vesicles as well as Proteasomes held together by a matrix protein. To discover the matrix protein holding ELVAs together w...
ORGANISM(S): Mus musculus (Mouse) 
2024-02-20 | PXD047716 | Pride
Elvas oocyte faps 
ProteomicsLC-MS of control vs BafA1-treated mouse oocytes during maturation
We discovered that mouse oocytes contain novel non-membrane-bound organelles, which we named EndoLysosomal Vesicular Assemblies (ELVAs). ELVAs contain endolysosomal and autophagy vesicles, as well as proteasomes. In immature oocytes, ELVAs store ubiquitinated proteins and show little degradative act...
ORGANISM(S): Mus musculus (Mouse) 
2024-02-20 | PXD042769 | Pride
Oocyte Lysosome Maturation Mouse Protein degradation 
ProteomicsDelivery of MFF through mitochondria-derived vesicles regulates melanosome maturation
Melanosomes are lysosome-associated organelles that produce and accumulate melanin. Their biogenesis begins with the trafficking of melanogenic proteins from the endo-lysosomal system into non-pigmented vacuoles derived from endosomes. Subsequently, melanosome maturation proceeds with the export and...
ORGANISM(S): Mus musculus (Mouse) 
2026-05-25 | PXD051372 | Pride
Mitochondria Mff Melanosome 
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