Sort   by:  
 Page size 
MetabolomicsQuantitative metabolomic analysis of changes in the lens and aqueous humor under development of age-related nuclear cataract

INTRODUCTION: Metabolites are essential for the proper functioning of the eye lens, they either enter the lens from the aqueous humor (AH), or are synthesized in the lens epithelium. Antioxidants, osmolytes and UV filters are especially important for the lens protection, and thei...

2022-11-07 | MTBLS740 | MetaboLights
TranscriptomicsBiochemical fractionation of HEK293 nuclei and RNA-seq of chromatin-associated and soluble-nuclear RNA
Biochemical fractionation of HEK293 nuclei and RNA-seq of chromatin-associated and soluble-nuclear RNA. Nuclei from three biological replicates were isolated by detergent lysis, fractionated, then three chromatin and three soluble RNA samples were converted to cDNA using Illumina TruSeq stranded pro...
ORGANISM(S): Homo sapiens 
2015-03-04 | E-GEOD-66478 | biostudies-arrayexpress
ProteomicsPTBP1 and PTBP2 associate with distinct proteins and have distinct post-translational modifications in nuclear neuronal extract.
PTBP1 and PTBP2 are highly conserved paralogs, yet exhibit distinct splicing patterns. Here we use mass spectrometry to show that PTBP1 and PTBP2 interact with distinct proteins and undergo distinct post translational modifications under splicing conditions in neuronal nuclear extract.
ORGANISM(S): Homo sapiens (Human) 
2026-02-09 | PXD058740 | Pride
Post-translational modificantions Ptbp1 Mass spectrometry Splicing Ptbp2 Protein-protein interactions 
TranscriptomicsNuclear scaffold and matrix attachment of Aortic Adventitial Fibroblast (AoAF) chromatin
The nuclear scaffold/matrix provides an anchor for higher order genome structure that has both structural and functional implications. Different extraction protocols, i.e., utilizing either 25 mM LIS or 2 M NaCl, isolate somewhat different protein constituents of either the nuclear scaffold or nucl...
ORGANISM(S): Homo sapiens 
2009-03-10 | E-GEOD-13792 | biostudies-arrayexpress
GenomicsC. elegans tissue after diet that includes nuclear and cystolic derived bovine thymus extract
C. elegans tissue after diet that includes nuclear and cystolic derived bovine thymus extract
PRJNA1137988 | ENA
ProteomicsProfile the composition of reconstituted nuclear speckles
Nuclear extract from Hela Cells are incubated with recombinant SON IDR2 protein, resulting condensates are spun down and the compostion profiled by mass spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2024-11-25 | PXD050371 | Pride
Nuclear speckles 
TranscriptomicsNuclear scaffold and matrix attachment of HeLa chromatin
The nuclear scaffold/matrix provides an anchor for higher order genome structure that has both structural and functional implications. Different extraction protocols, i.e., utilizing either 25 mM LIS or 2 M NaCl, isolate somewhat different protein constituents of either the nuclear scaffold or nucl...
ORGANISM(S): Homo sapiens 
2010-05-22 | E-GEOD-13774 | biostudies-arrayexpress
GenomicsReprogramming of fish somatic cells for nuclear transfer is primed by Xenopus egg extract
Reprogramming of fish somatic cells for nuclear transfer is primed by Xenopus egg extract
PRJNA847954 | ENA
TranscriptomicsNuclear Matrix Association in Proliferative and Invasive Cytotrophoblasts
Abnormal trophoblast invasion is associated with the most common and most severe complications of human pregnancy. The biology of invasion, as well as the etiology of abnormal invasion remains poorly understood. The aim of this study was to characterize the transcriptome of the HTR-8/SVneo human c...
ORGANISM(S): Homo sapiens 
2010-06-22 | E-GEOD-17501 | biostudies-arrayexpress
GenomicsWhole Cell Extract
Most human genes are loaded with promoter proximally paused RNA polymerase II (RNAP II) molecules that are poised for release into productive elongation by P-TEFb. Gdown1, a protein that renders RNAP II responsive to mediator, is involved in RNAP II elongation control. During in vitro transcription ...
ORGANISM(S): Homo sapiens 
2012-02-21 | E-GEOD-35882 | biostudies-arrayexpress
Sort   by:  
 Page size