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ProteomicsCedA: A General Transcription Factor from E. coli with a Distinct Mechanism of Action
Gene expression in E. coli is controlled by well-established mechanisms that activate or repress transcription. Here, we identify CedA as an unconventional transcription factor specifically associated with the RNA polymerase (RNAP) σ70 holoenzyme. Structural and biochemical analysis of CedA bound to...
ORGANISM(S): Escherichia coli Bacteria 
2023-09-17 | PXD039446 | Pride
Transcription Initiation factor Rna polymerase Antibiotic resistance 
GenomicsEscherichia coli
Genome-wide analysis of transcriptional termination in E. coli
PRJNA105589 | ENA
ProteomicsRapid immunoprecipitation and mass spectrometry of endogenous proteins on chromatin (RIME) analysis of androgen receptor and 14-3-3 ζ in PIM1 over-expressing LNCaP cells
14-3-3 ζ is an androgen receptor (AR) co-activator. To further understand how 14-3-3 ζ may be modulating AR activity, we performed rapid immunoprecipitation mass spectrometry of endogenous proteins for analysis of chromatin complexes (RIME) on AR and 14-3-3 ζ in LNCaP prostate cancer cells in the pr...
ORGANISM(S): Homo sapiens (Human) 
2021-11-25 | PXD023634 | Pride
Protein interactions Prostate cancer Pim1 14-3-3 ζ Androgen receptor 
ProteomicsPIM1 phosphorylation of 14-3-3 ζ in vitro
We previously identified 14-3-3 ζ S64 as a substrate of PIM1 in an in vivo chemical genetic screen. To verify this phosphorylation event and determine whether S64 is the predominant phosphorylation site, we performed in vitro phosphorylation of 14-3-3 ζ by PIM1.
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2021-11-25 | PXD023623 | Pride
Pim1 Protein phosphorylation Kinase 14-3-3 ζ 
ProteomicsThe structure of a virus-encoded nucleosome
Eukaryotes and prokaryotes encode proteins with the histone-fold domain, but only eukaryotes are known to encode "core histones" from four distinct families that heterodimerize selectively and uniquely to form nucleosome particles. Some large DNA viruses, including those in Marseilleviridae family e...
ORGANISM(S): Marseillevirus 
2021-05-27 | PXD024220 | Pride
Chromatin Marseillevirus Nucleosomes Cryo-em Evolution Histones 
ProteomicsTargeting the fitness cost of antibiotic resistance via the non-essential genome
Mutations in the rifampicin (Rif)-binding site of RNA polymerase (RNAP) impart antibiotic resistance and inextricably affect transcription initiation, elongation, and termination properties as well. At each step of the transcription cycle, RNAP responds to non-essential transcription factors, signal...
ORGANISM(S): Escherichia coli 
2021-11-25 | PXD027810 | Pride
Fitness cost Antibiotic resistance Rifampicin Rna polymerase 
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