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ProteomicsA nowhere-to-hide mechanism ensures complete piRNA-directed LINE1 methylation
The mouse PIWI-interacting RNA (piRNA) pathway provides anti-transposon immunity to the developing male germline by directing transposon DNA methylation. The first step in this process is the recruitment of SPOCD1 to young LINE1 loci 1 followed in the second step by piRNA-mediated tethering of the P...
ORGANISM(S): Mus musculus (Mouse) 
2025-11-11 | PXD060851 | Pride
Piwi proteins Pirna Spocd1 and tpr Germline Transposons 
ProteomicsTEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing.
The PIWI protein MIWI2 and its associated PIWI-interacting RNAs (piRNAs) instruct DNA methylation of young active transposable elements (TEs) in the male germline. Here we show that MIWI2 associates with TEX15 in foetal gonocytes. TEX15 is predominantly a nuclear protein that is not required for piR...
ORGANISM(S): Mus musculus (Mouse) 
2020-06-18 | PXD019087 | Pride
Mouse transposon silencing 
TranscriptomicsDefective germline reprogramming rewires the spermatogonial transcriptome
Defective germline reprogramming in Miwi2- and Dnmt3l-deficient mice results in the failure to reestablish transposon silencing, meiotic arrest and progressive loss of spermatogonia. Here we sought to understand the molecular basis for this spermatogonial dysfunction. Through a combination of imagin...
ORGANISM(S): Mus musculus 
2018-03-09 | E-MTAB-6202 | biostudies-arrayexpress
TranscriptomicsAffymetrix gene expression array (mouse 2.0) of sorted undifferentiated spermatogonia in inducible Miwi2 knock-out model
Undifferentiated spermatogonia were sorted from Miwi2Tom/FL; ERT-Cre/+ (Miwi2-iKO) and Miwi2Tom/+; ERT-Cre/+ (Miwi2-CTL) mice injected with tamoxifen two weeks after last tamoxifen injection to understand an impact of acute Miwi2 deletion on spermatogenic homeostasis.
ORGANISM(S): Mus musculus 
2018-03-21 | E-MTAB-6612 | biostudies-arrayexpress
GenomicsTEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing.
The PIWI protein MIWI2 and its associated PIWI-interacting RNAs (piRNAs) instruct DNA methylation of young active transposable elements (TEs) in the male germline. Here we show that MIWI2 associates with TEX15 in foetal gonocytes. TEX15 is predominantly a nuclear protein that is not required for piR...
ORGANISM(S): Mus musculus 
2020-06-17 | E-MTAB-9090 | biostudies-arrayexpress
ProteomicsA nowhere-to-hide mechanism ensures complete piRNA-directed LINE1 methylation
The mouse PIWI-interacting RNA (piRNA) pathway provides anti-transposon immunity to the developing male germline by directing transposon DNA methylation. The first step in this process is the recruitment of SPOCD1 to young LINE1 loci 1 followed in the second step by piRNA-mediated tethering of the P...
ORGANISM(S): Mus musculus (Mouse) 
2025-11-11 | PXD060850 | Pride
Piwi proteins Pirna Spocd1 and tpr Germline Transposons 
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