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Proteomics‘One-pot’ sample processing method for proteome-wide analysis of microbial cells and spores
Bacterial endospores, the transmissible forms of pathogenic bacilli and clostridia, are heterogeneous multilayered structures composed of proteins. These proteins protect the spores against variety of stresses, thus helping spore survival, and assist in germination, by interacting with the environme...
ORGANISM(S): Bacillus cereus (strain ATCC 14579 / DSM 31) Bacillus subtilis PY79 Peptoclostridium difficile (strain 630) (Clostridium difficile) 
2018-07-05 | PXD008242 | Pride
Spores One-pot method Zic-hilic Proteomics Mass spectrometry 
ProteomicsSurfactant-assisted one-pot sample preparation for label-free single-cell proteomics
Large numbers of cells are generally required for quantitative global proteome profiling due to the significant surface adsorption losses associated with sample processing. Such bulk measurement obscures important cell-to-cell variability (cell heterogeneity) and makes proteomic profiling impossible...
ORGANISM(S): Homo Sapiens 
2021-03-11 | PXD022827 | panorama
MetabolomicsTwo apples a day modulate human:microbiome co-metabolic processing of polyphenols, tyrosine and tryptophan

PURPOSE: Validated biomarkers of food intake (BFIs) have recently been suggested as a useful tool to assess adherence to dietary guidelines or compliance in human dietary interventions. Although many new candidate biomarkers have emerged in the last decades for different foods fr...

2020-04-28 | MTBLS469 | MetaboLights
ProteomicsUltrasensitive Top-Down Proteomics Analysis of Low Number of Mammalian Cells Using a Nanodroplet Sample Processing Platform
We developed an ultrasensitive top down platform by incorporating a microfluidic sample preparation system, termed nanoPOTS (Nanodroplet Processing in One pot for Trace Samples), into a top down workflow. We determined that a unique combination of a nonionic detergent DDM with urea as a protein extr...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-11-22 | MSV000084612 | MassIVE
ProteomicsFacile one-pot nanoproteomics for label-free proteome profiling of 50-1000 mammalian cells
We report a facile one-pot nanoproteomics method termed SOPs-MS (Surfactant-assisted One-Pot sample processing at the standard volume coupled with MS) for convenient proteome profiling of 50-1000 mammalian cells. Building upon our recent development of SOP-MS for label-free single-cell proteomics at...
ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse) 
2022-05-11 | PXD025918 | JPOST Repository
ProteomicsFacile one-pot nanoproteomics for label-free proteome profiling of 50-1000 mammalian cells
We report a facile one-pot nanoproteomics method termed SOPs-MS (Surfactant-assisted One-Pot sample processing at the standard volume coupled with MS) for convenient proteome profiling of 50-1000 mammalian cells. Building upon our recent development of SOP-MS for label-free single-cell proteomics at...
ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse) 
2021-07-23 | PXD026009 | JPOST Repository
ProteomicsRapid, One-step Sample Processing for Label-Free Single-Cell Proteomics
Sample preparation for single-cell proteomics is generally performed in a one-pot workflow with multiple dispensing and incubation steps. These hours-long workflows can be labor intensive and lead to long sample-to-answer times. Here we report a sample processing method that achieves cell lysis, pro...
ORGANISM(S): Homo sapiens (Human) 
2023-10-09 | PXD041879 | Pride
Sample preparation Single-cell proteomics Lc-msms 
ProteomicsSpatially resolved top-down proteomics of tissue sections based on a microfluidic nanodroplet sample preparation platform
We demonstrate spatially resolved top-down proteomics (TDP) for proteoform identification and quantification from small sections of rat brain. We used the nanoPOTS (nanodroplet Processing in One pot for Trace Samples) platform, further improving the sample preparation by adding benzonase in the extr...
ORGANISM(S): Rattus Norvegicus (ncbitaxon:10116) Homo Sapiens (ncbitaxon:9606) 
2022-03-30 | MSV000089163 | MassIVE
UnknownSurfactant-assisted one-pot sample preparation for label-free single-cell proteomics.
Not available
S-EPMC7921383 | biostudies-literature
ProteomicsOne-Pot Isotope Dilution PRM for Quantitative Protein Heterogeneity in Red Blood Cells
Single cell and low-input cell proteomics to measure cellular heterogeneity remains experimental and requires further refinement for routine application. This study aimed to build on previous protocols to assess the utility of targeted proteomics for measuring heterogeneity in red blood cells (RBCs)...
ORGANISM(S): Homo Sapiens 
2024-02-06 | PXD030875 | panorama
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