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UnknownRevealing nascent proteomics in signaling pathways and cell differentiation.
Not available
S-EPMC5877968 | biostudies-literature
UnknownCapture, Release, and Identification of Newly Synthesized Proteins for Improved Profiling of Functional Translatomes.
Not available
S-EPMC9971285 | biostudies-literature
ProteomicsBone Marrow Mesenchymal Stromal Cells Support Translation in Refractory Acute Myeloid Leukemia
In acute myeloid leukemia (AML), malignant cells surviving chemotherapy rely on high mRNA translation and their microenvironmental metabolic support to drive relapse. However, the role of translational reprogramming in the niche is unclear. Here we found that relapsing AML cells increase translation...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2024-12-27 | PXD058646 | Pride
Aml niche Translational reprogramming 
ProteomicsPuromycin acetyltransferase-mediated cell-specific labeling of de novo synthesized proteins, part I
Puromycin and its derivative O-propargyl puromycin (OPP) enable detection of nascent proteins. Use of these metabolic labels in complex mixtures of cells leads to indiscriminate tagging of nascent proteomes independent of cell type. Here we have used puromycin N-acetyltransferase (PAT), for cell-spe...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2023-10-24 | PXD043494 | Pride
Neuro2a Hek Opp-id Shotgun proteomics Streptavidin pulldown 
ProteomicsPuromycin acetyltransferase-mediated cell-specific labeling of de novo synthesized proteins, Part II
Puromycin and its derivative O-propargyl puromycin (OPP) enable detection of nascent proteins. Use of these metabolic labels in complex mixtures of cells leads to indiscriminate tagging of nascent proteomes independent of cell type. Here we have used puromycin N-acetyltransferase (PAT), for cell-spe...
ORGANISM(S): Rattus rattus (Black rat) 
2023-10-24 | PXD043498 | Pride
Opp-id Shotgun proteomics Streptavidin pulldown Rat cortical neuron-glia co-culture 
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