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TranscriptomicsRNA-seq of human embryonic stem cells during cell cycle progression
The experiment was designed to look into transcriptional changes during cell cycle progression in human embryonic stem cells (hESCs). For this, FUCCI hESCs are sorted in Early G1 (EG1), Late G1 (LG1), and S/G2/M phases in triplicates, RNA was extracted and PolyA purified libraries were generated and...
ORGANISM(S): Homo sapiens 
2023-06-24 | E-MTAB-8347 | biostudies-arrayexpress
TranscriptomicsATAC-seq of human embryonic stem cells during cell cycle progression
The experiment was designed to look into chromatin accessibility changes during cell cycle progression in human embryonic stem cells (hESCs). For this, FUCCI hESCs are sorted in Early G1 (EG1), Late G1 (LG1), G1/S transition and S/G2/M phases in duplicates. 100,000 were used per sample, as described...
ORGANISM(S): Homo sapiens 
2023-06-24 | E-MTAB-8348 | biostudies-arrayexpress
GenomicsChIP-seq of human embryonic stem cells during cell cycle progression
The experiment was designed to look into changes in key transcription factors (TFs) binding sites during cell cycle progression in human embryonic stem cells (hESCs). For this, 1 million FUCCI hESCs are sorted in Early G1 (EG1), Late G1 (LG1), and S/G2/M phases in duplicates for each experiment, and...
ORGANISM(S): Homo sapiens 
2023-06-24 | E-MTAB-8358 | biostudies-arrayexpress
TranscriptomicsRNA-seq of WTAP knockdown human pluripotent stem cells during the exit from pluripotency induced by Activin/Nodal inhibition
This experiment was designed to monitor the gene expression changes induced by the knockdown of WTAP in human pluripotent stem cells (hPSCs) during the exit from pluripotency induced by the inhibition of Activin/Nodal signalling. hPSCs carrying a tetracycline-inducible short hairpin RNA (shRNA) agai...
ORGANISM(S): Homo sapiens 
2018-01-04 | E-MTAB-5230 | biostudies-arrayexpress
TranscriptomicsSingle cell RNA sequencing of naive and primed human pluripotent stem cells treated with SB431542
The aim of this experiment was to investigate the role of TGFβ signalling in human pluripotency, looking at the effect of SB431542 (SB), a potent and selective SMAD inhibitor that blocks TGFβ/Activin receptors ALK5, ALK4, and ALK7, on gene expression. We performed 10X sequencing in naïve and primed ...
ORGANISM(S): Homo sapiens 
2021-08-13 | E-MTAB-10018 | biostudies-arrayexpress
GenomicsSMAD2/3 chromatin immunoprecipitation sequencing (ChIP-seq) in human naïve and primed human pluripotent stem cells
The aim of this experiment was to investigate the role of TGFβ signalling pathway in human pluripotency, through ChIP-seq analysis of its main downstream effector SMAD2/3 in naïve and primed human pluripotent stem cells (hPSCs).
ORGANISM(S): Homo sapiens 
2021-08-13 | E-MTAB-10017 | biostudies-arrayexpress
TranscriptomicsRNA-seq of definitive endoderm differentiation of human embryonic stem cells in early G1 phase of the cell cycle
The experiment was designed to investigate transcriptional dynamics during definitive endoderm differentiation of human embryonic stem cells (hESCs) in early G1 phase of the cell cycle. For this, the FUCCI system was used to sort hESCs in Early G1 (EG1). Samples were taken at 0h (hESCs), 12h, 24h, 3...
ORGANISM(S): Homo sapiens 
2020-09-07 | E-MTAB-9194 | biostudies-arrayexpress
TranscriptomicsActivin/Nodal-dependent N6-methyladenosine (m6A) deposition in human pluripotent stem cells
This experiment was designed to probe the function of Activin/Nodal signalling in the deposition of m6A in human pluripotent stem cells (hPSCs). hPSCs were cultured in presence of Activin or subjected to short-term inhibition of Activin/Nodal signalling for 2h using the receptor antagonist SB-431542...
ORGANISM(S): Homo sapiens 
2017-12-26 | E-MTAB-5229 | biostudies-arrayexpress
UnknownSerum coating enables feeder-free culture of naive human pluripotent stem cells preserving developmental potential
Not available
S-SCDT-10_1038-S44318-026-00714-2 | biostudies-other
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