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ProteomicsCrosslinking mass spectrometry of the p300 catalytic core complexed with the nucleosome
p300 is a histone acetyltransferase that associates with crucial biological processes. p300 acetylates all four histones in the nucleosome, a basic unit of chromatin, and alters chromatin structure and dynamics. In this study, we performed structural and biochemical analysis to understand the nucleo...
ORGANISM(S): Homo Sapiens (human) 
2022-06-29 | PXD033804 | JPOST Repository
TranscriptomicsTranscription profiling of mouse C2AS12 cells infected with (1) wt p300 + tTA, (2) p300deltaTAZ2 +tTA, (3) wt p300 +tTA +doxycycline to define the transcriptional network regulated by ectopic expression of the transcriptional co-activator protein, p300 and define its role in promoting muscle cell survival
The primary focus of these studies is to define the transcriptional network regulated by ectopic expression of the transcriptional co-activator protein, p300, in order to define its mechanism(s) of action in promoting muscle cell survival. Our laboratory has generated a murine C2-derived myoblast ce...
ORGANISM(S): Mus musculus 
2007-12-07 | E-GEOD-4224 | biostudies-arrayexpress
ProteomicsTime-resolved analysis reveals rapid dynamics and broad scope of the CBP/p300 acetylome
The acetyltransferases CBP and p300 are multifunctional transcriptional co-activators; however, their acetylation targets, site-specific acetylation kinetics, and function in proteome regulation are incompletely understood. We combined quantitative proteomics with novel CBP/p300-specific catalytic i...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
MSV000095981 | MassIVE
ProteomicsKcr proteome in response to p300 KO
We used quantitative proteomics to characterize the p300-regulated lysine crotonylome in wild type (WT) and p300 knockout (KO) cells.
ORGANISM(S): Homo sapiens (Human) 
2018-06-26 | PXD006407 | Pride
Crotonylation P300 Quantitative proteomics 
ProteomicsNuclear condensates of p300 formed though the structured catalytic core can act as a storage pool of p300 with reduced HAT activity
The transcriptional co-activator and acetyltransferase p300 is required for fundamental cellular processes, including differentiation and growth. Here, we report that p300 forms phase separated condensates in the cell nucleus. The phase separation ability of p300 is regulated by autoacetylation and ...
ORGANISM(S): Homo sapiens (Human) 
2021-08-26 | PXD026898 | Pride
P300 nuclear condensates hat activity 
GenomicsChIP-seq Accurately Predicts Tissue-Specific Activity of Enhancers
Determining the spatial and temporal activity patterns of enhancers remains a challenge in the functional annotation of the human genome. Here, we performed genome-wide mapping of tissue-specific in vivo binding sites for the enhancer-associated protein p300 and assessed in transgenic mice the util...
ORGANISM(S): Mus musculus 
2009-02-11 | E-GEOD-13845 | biostudies-arrayexpress
ProteomicsKhib proteome in response to p300 KO
We used quantitative proteomics to characterize the p300-regulated lysine 2-hydroxyisobutyrylome in wild type (WT) and p300 knockout (KO) cells.
ORGANISM(S): Homo sapiens (Human) 
2018-07-11 | PXD008525 | Pride
P300 Lysine 2-hydroxyisobutyrylation 
ProteomicsTargeted degradation of the enhancer lysine acetyltransferases CBP and p300
The enhancer factors CBP/p300 maintain gene expression programs through lysine acetylation of chromatin and transcriptional regulators and by scaffolding functions mediated by several protein-protein interaction domains. We have designed dCBP-1, a compound that induces degradation of CBP/p300, and ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-11-15 | MSV000086457 | MassIVE
OtherData for: Short-chain fatty acids activate acetyltransferase p300/CBP
The short-chain fatty acids (SCFAs) acetate, propionate, and butyrate are produced in large quantities by the gut microbiome and contribute to a wide array of physiological processes. While the underlying mechanisms are largely unknown, many effects of SCFAs have been traced to changes in epigenetic...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-07-12 | MSV000087800 | MassIVE
GenomicsGenome-wide assessment of differential roles for p300 and CBP in transcription regulation
Through ChIP-Seq analysis with the Illumina Whole Genome Analyzer, we identify binding sites for P300 (EP300) and CBP (CREBBP) in Human glioblastoma T98G cells that were cell cycle synchronized before and after stimulation. In our analysis, we focused on the identification of genes differentially bo...
ORGANISM(S): Homo sapiens 
2010-03-30 | E-GEOD-21026 | biostudies-arrayexpress
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