Sort   by:  
 Page size 
ProteomicsFACT, PAF-C, and CKII
Protein-protein interaction analysis for RNA Polymerase II and its elongation factors including: the PAF Complex, the FACT Complex, casein kinase II, DSIF, and TFIIF. These studies also characterized a number of casein kinase II phosphorylation sites on the subunits of the PAF Complex.
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2015-07-20 | MSV000079197 | MassIVE
GenomicsThe PAF complex regulates alternative splicing.
The PAF complex regulates alternative splicing.
PRJNA1052729 | ENA
TranscriptomicsPaf1C plays novel subunit-specific roles in alternative cleavage and polyadenylation
The PAF complex (Paf1C) has been shown to regulate chromatin modifications, gene transcription, and PolII elongation. Here, we provide the first genome-wide analysis of chromatin occupancy by the entire PAF complex in mammalian cells. We show that Paf1C is recruited not only to promoters and gene bo...
ORGANISM(S): Mus musculus 
2016-01-15 | E-GEOD-72574 | biostudies-arrayexpress
GenomicsThe Mediator complex subunit MED23 couples H2B mono-ubiquitination to transcriptional control and cell fate determination
The Mediator complex orchestrates multiple transcription factors with the Pol II apparatus for precise transcriptional control. However, its interplay with the surrounding chromatin remains poorly understood. Here, we analyze differential histone modifications between WT and MED23-/- (KO) cells and ...
ORGANISM(S): Mus musculus 
2015-09-16 | E-GEOD-59518 | biostudies-arrayexpress
TranscriptomicsInfluence of alkyl-phospholipids on the gene expression profile of immortalized keratinocytes HaCaT
New alkyl-phospholipids (APLs) that are structurally derived from the platelet-activating factor are promising candidates for anticancer treatment. After the incorporation into cell membranes, APLs are able to interfere with a wide variety of key enzymes implicated in cell growth, motility, invasion...
ORGANISM(S): Homo sapiens 
2011-03-01 | E-GEOD-20722 | biostudies-arrayexpress
TranscriptomicsThe PAF complex regulates alternative splicing.
The conserved Eukaryotic PAF complex binds to transcribing RNA polymerase II to control deposition of histone marks during transcription. Recently its role in alternative polyadenylation (selection of mRNA 3’end cleavage sites by CPSF) was described. In this work we show that the PAF complex also re...
ORGANISM(S): Homo sapiens 
2024-11-05 | GSE250246 | GEO
GenomicsLeo1 is a Global Regulator of Heterochromatin Cis-spreading
Domains of heterochromatin play important roles in the maintenance and regulation of eukaryotic genomes. However, the repressive nature of heterochromatin combined with its propensity to self-propagate necessitates the existence of robust mechanisms that limit heterochromatin spreading and thereby a...
ORGANISM(S): Schizosaccharomyces pombe 
2015-05-22 | E-GEOD-61688 | biostudies-arrayexpress
GenomicsUbiquitin-dependent turnover of MYC promotes loading of the PAF complex on RNA Polymerase II to drive transcriptional elongation (ChIP-seq)
The MYC transcription factor is an unstable protein and its turnover is controlled by the ubiquitin system. Ubiquitination enhances MYC-dependent transactivation, but the underlying mechanism remains unresolved. Here we show that proteasomal turnover of MYC is dispensable for recruitment of RNA poly...
ORGANISM(S): Homo sapiens 
2016-01-04 | E-GEOD-70001 | biostudies-arrayexpress
TranscriptomicsUbiquitin-dependent turnover of MYC promotes loading of the PAF complex on RNA Polymerase II to drive transcriptional elongation (RNA-seq)
The MYC transcription factor is an unstable protein and its turnover is controlled by the ubiquitin system. Ubiquitination enhances MYC-dependent transactivation, but the underlying mechanism remains unresolved. Here we show that proteasomal turnover of MYC is dispensable for recruitment of RNA poly...
ORGANISM(S): Homo sapiens 
2016-01-04 | E-GEOD-70000 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of S. cerevisiae paf1 and ctr9 mutants to identify direct targets of the Paf1/RNA polymerase II complex
To identify the direct targets of the Paf1/RNA polymerase II complex we compared expression profiles of isogenic wild type and paf1 and ctr9 mutant strains. We also created a Tet-regulated form of Paf1 and monitored expression patterns after shut off of Paf1. Samples were isolated at one hour interv...
ORGANISM(S): Saccharomyces cerevisiae 
2007-09-19 | E-GEOD-3200 | biostudies-arrayexpress
Sort   by:  
 Page size