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MetabolomicsMetabolic responses induced by DNA damage and poly (ADP-ribose) polymerase (PARP) inhibition in MCF-7 cells
Genomic instability is one of the hallmarks of cancer. Several chemotherapeutic drugs and radiotherapy induce DNA damage to prevent cancer cell replication. Cells in turn activate different DNA damage response (DDR) pathways to either repair the damage or induce cell death. These DDR pathways also e...
2015-08-01 | MTBLS177 | MetaboLights
ProteomicsChemical genetics and proteome-wide site mapping reveal cysteine MARylation by PARP-7 on immune-relevant protein targets
Poly (ADP-ribose) polymerase 7 (PARP-7) has emerged as a critically important member of a large enzyme family that catalyze ADP-ribosylation in mammalian cells. PARP-7 is a critical regulator of the innate immune response. What remains unclear is the mechanism by which PARP-7 regulates this process,...
ORGANISM(S): Homo sapiens (Human) 
2021-07-21 | PXD020323 | Pride
Chemical genetics Marylation Parp-7 Protein-protein interactions Enzymatic targets 
TranscriptomicsRNAi of human MCF7 cells knocked down for PARP-1 or PARGs
Poly(ADP-ribose) polymerase-1 (PARP-1) and poly(ADP-ribose) glycohydrolase (PARG) are enzymes that modify target proteins in the nucleus by the addition and removal, respectively, of ADP-ribose polymers. Although a role for PARP-1 in gene regulation has been well established, the role of PARG is le...
ORGANISM(S): Homo sapiens 
2009-10-30 | E-GEOD-12952 | biostudies-arrayexpress
TranscriptomicsMCF-7 Luciferase, PARP-1, PARG, SIRT1, and macroH2A Knockdown
Affymetrix expression arrays were used to compare expression patterns upon knockdown of PARP-1, PARG, SIRT1, or macroH2A in comparison to Luciferase control. Total RNA isolated from three independently generated control-matched MCF-7 knockdown cell lines targeting Luciferase (control), PARP-1, PARG...
ORGANISM(S): Homo sapiens 
2010-02-21 | E-GEOD-12971 | biostudies-arrayexpress
ProteomicsSubstrate Identification Using a Chemical Genetics Approach Reveals a Role for PARP-7-Mediated MARylation in Controlling Microtubule Stability in Ovarian Cancer Cells
To identify the ADPRylated substrates that underlie PARP-7-mediated ovarian cancer cell phenotypes, we developed an NAD+ analog-sensitive approach for PARP-7 comprising a PARP-7 NAD+ binding pocket mutant (S563G) paired with the NAD+ analog 8-Bu(3-yne)T-NAD+. When coupled with mass spectrometry, th...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-12-17 | MSV000086611 | MassIVE
TranscriptomicsExamination of functional interplay between NMNAT-1 (a nuclear NAD+ synthase) and PARP-1 (a nuclear poly(ADP-ribose) polymerase)
NMNAT-1 and PARP-1, two key enzymes in the NAD+ metabolic pathway, localize to the nucleus where integration of their enzymatic activities has the potential to control a variety of nuclear processes. Using a variety of biochemical, molecular, cell-based, and genomic assays, we show that NMNAT-1 and...
ORGANISM(S): Homo sapiens 
2012-02-15 | E-GEOD-35358 | biostudies-arrayexpress
UnknownMolecular Mechanisms of PARP-1 Inhibitor 7-Methylguanine.
Not available
S-EPMC7139824 | biostudies-literature
TranscriptomicsExpression Analysis Upon PARP-1 or PARG Knockdown in MCF-7 Cells
Poly(ADP-ribose) polymerase-1 (PARP-1) and poly(ADP-ribose) glycohydrolase (PARG) are enzymes that modify target proteins in the nucleus by the addition and removal, respectively, of ADP-ribose polymers. Although a role for PARP-1 in gene regulation has been well established, the role of PARG is les...
ORGANISM(S): Homo sapiens 
2009-10-23 | GSE12952 | GEO
GenomicsSubstrate Identification Using a Chemical Genetics Approach Reveals a Role for PARP-7-Mediated MARylation in Controlling Microtubule Stability in Ovarian Cancer Cells
Substrate Identification Using a Chemical Genetics Approach Reveals a Role for PARP-7-Mediated MARylation in Controlling Microtubule Stability in Ovarian Cancer Cells
PRJNA642215 | ENA
TranscriptomicsSubstrate Identification Using a Chemical Genetics Approach Reveals a Role for PARP-7-Mediated MARylation in Controlling Microtubule Stability in Ovarian Cancer Cells
ADP-ribosylation (ADPRylation) is a regulatory posttranslational modification of proteins that results in the covalent attachment of ADP-ribose (ADPR) moieties on target proteins. PARP-7 (TiPARP) is a monoADPR transferase whose proteins substrates and biological activities are poorly understood. Her...
ORGANISM(S): Homo sapiens 
2021-02-04 | GSE153395 | GEO
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