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ProteomicsMass Spectrometry Analysis of Histone Modifications in Sorghum during Drought Acclimation
This bottom-up analysis is the supplementary data file to the top-down analysis deposited with identifier PXD014660. The peptide data are used to support assignments of modifications detected in top-down. Details are described in the associated manuscript.
ORGANISM(S): Sorghum bicolor 
2019-10-30 | PXD015508 | Pride
Post-translational modifications Mass spectrometry Plant Epigenetics Histone clipping 
ProteomicsTop-Down Mass Spectrometry Analysis of Histone Modifications in Sorghum during Drought Acclimation
This study explores the changes in histone modifications of sorghum bicolor through developmental stages and in response to drought stress in two sorghum genotypes. We analyzed the leaves of 48 plants using top-down mass spectrometry and identified 26 unique histone proteins and 677 unique histone p...
ORGANISM(S): Sorghum bicolor 
2019-10-30 | PXD014660 | Pride
Post-translational modifications Mass spectrometry Top-down Plant Epigenetics Histone clipping 
MetabolomicsDecreased abundance of type III secretion systeminducing signals in Arabidopsis mkp1 enhances resistance against Pseudomonas syringae
Genes encoding the virulence-promoting type III secretion system (T3SS) in phytopathogenic bacteria are induced at the start of infection, indicating that recognition of signals from the host plant initiates this response. However, the precise nature of these signals and whether their concentrations...
2014-06-24 | MTBLS95 | MetaboLights
ProteomicsAn improved Boosting to Amplify Signal with Isobaric Labeling (iBASIL) strategy for precise quantitative single-cell proteomics
Mass spectrometry (MS)-based proteomics has great potential for overcoming the limitations of antibody-based immunoassays for antibody-independent, comprehensive, and quantitative proteomic analysis of single cells. Indeed, recent advances in nanoscale sample preparation have enabled effective proce...
ORGANISM(S): Homo Sapiens (human) 
2020-03-05 | PXD017626 | JPOST Repository
ProteomicsHigh-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chip
Development and evaluation of nested nanowell (N2) chip in single-cell proteomics. The newly designed chip helps to improve the sensitivity and robustness of the tandem mass tag labeling approach by sizing down wells.
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2021-02-04 | MSV000086809 | MassIVE
ProteomicsAn intelligent Boosting to Amplify Signal with Isobaric Labeling (iBASIL) strategy for precise quantitative single-cell proteomics
Mass spectrometry (MS)-based proteomics has great potential for overcoming the limitations of antibody-based immunoassays for antibody-independent, comprehensive, and quantitative proteomic analysis of single cells. Indeed, recent advances in nanoscale sample preparation have enabled effective proce...
ORGANISM(S): Homo Sapiens (human) 
2020-03-05 | PXD016057 | JPOST Repository
ProteomicsLaser capture microdissected functional tissue units from kidney for top-down proteomic characterization using microPOTS
Human kidney tissue obtained from Vanderbilt University Biomolecular Multimodal Imaging Center (BIOMIC) was laser capture microdissected onto microPOTS chips. Six replicates of 100,000 um2 from three distinct functional tissue units (glomeruli, tubules, and medullary rays) were acquired from a 10 um...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-12-22 | MSV000093722 | MassIVE
ProteomicsAutomated coupling of nanodroplet sample preparation with liquid chromatography-mass spectrometry for high throughput single-cell proteomics
Development and evaluation of an autosampler for integrating nanoPOTS with LC-MS. Includes proteomic data from nanoPOTS-based sample preparation, including diluted peptides of Shewanella oneidensis MR-1, single cultured MCF10A cells, and single cells from three Acute Myeloid Leukemia (AML) cell line...
ORGANISM(S): Shewanella Oneidensis Mr-1 (ncbitaxon:211586) Homo Sapiens (ncbitaxon:9606) 
2020-04-05 | MSV000085230 | MassIVE
ProteomicsHigh-Throughput Single Cell Proteomics Enabled by Multiplex Isobaric Labeling in a Nanodroplet Sample Preparation Platform
Single cell proteomics data from three murine cell lines (Raw, SVEC4-10, C10) and HeLa cell lysate. Samples were digested with trypsin and labeled with TMT 10-Plex on a small scale using nanoPOTS, followed by LC-MS/MS analysis.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
2019-07-19 | MSV000084110 | MassIVE
ProteomicsAnalysis of protein phosphorylation in response to the ATP treatment in Arabidopsis thaliana
The project intended to reveal protein phosphorylation patterns in Arabidopsis thaliana in response to ATP. For this purpose, Arabidopsis thaliana plants, including WT, ATP receptor mutants (p2k1, p2k2, and double mutant p2k1/p2k2), and P2K1 overexpression plants, were treated with ATP or buffer (as...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
MSV000095888 | MassIVE
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