Pauklin Siim S - OmicsDI

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Homo sapiens (2)
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biostudies-arrayexpress (2)
pride (2)

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Pancreas (1)
Pancreatic ductal adenocarcinoma cell line (1)

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Pancreatic ductal adenocarcinoma (1)

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Mass Spectrometry (2)
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2020 (1)
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Modification

2x(15)N labeled L-lysine (1)
4x(15)N labeled L-arginine (1)

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ChIP-seq (1)
RNA-seq of coding RNA (1)

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2020 (1)
2016 (1)

Lab affiliation

Botnar Research Centre, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, Old Road, University of Oxford, Oxford OX3 7LD, UK (1)
Botnar Research Centre, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, UK (1)

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RNA-seq of definitive endoderm differentiation of human embryonic stem cells in early G1 phase of the cell cycle

The experiment was designed to investigate transcriptional dynamics during definitive endoderm differentiation of human embryonic stem cells (hESCs) in early G1 phase of the cell cycle. For this, the FUCCI system was used to sort hESCs in Early G1 (EG1). Samples were taken at 0h (hESCs), 12h, 24h, 3...

ORGANISM(S): Homo sapiens

2020-09-07 | E-MTAB-9194 | biostudies-arrayexpress

Cyclin D1 ChIP-seq in H9 undifferentiated human pluripotent stem cells

H9 human pluripotent stem cells were grown in chemically defined media with Activin A and FGF2. Four 10cm plates with 70% confluency were used per sample. Cells were cross-linked by adding a final concentration of 1% PFA into culture media and incubated for 10 min at RT for Cyclin D1 ChIP. ChIP-seq ...

ORGANISM(S): Homo sapiens

2016-02-01 | E-MTAB-3807 | biostudies-arrayexpress

Targeting an RNA polymerase II-associated PHF5A protein subcomplex with a KMT2A-WDR5 inhibitor impairs self-renewal and tumorigenicity of pancreatic cancer stem cells

Pancreatic cancer (PC) remains one of the most aggressive and life-threatening malignancies known for its notorious resistance to chemotherapy. This is increasingly ascribed to the subpopulation of undifferentiated cells, known as pancreatic cancer stem cells (PCSCs), which are evolutionary fitter t...

ORGANISM(S): Homo sapiens (Human)

2023-08-23 | PXD038378 | Pride

Quantitative secretomics identifies ligand-receptor pairs in pancreatic cancer that mediate bidirectional crosstalk between cancer stem cells and nociceptors

Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest and most metastatic cancers in human. PDACs respond poorly to therapies, partly due to cancer stem cells (CSCs) that self-renew, survive chemotherapies, metastasise and replenish the tumour. Factors secreted by tumour cells mediate auto...