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ProteomicsConstruction, debugging and iterative redesign of synXVI, a 903-kb synthetic chromosome of Saccharomyces cerevisiae
Assembly and debugging of the 902,994-bp synthetic Saccharomyces cerevisiae chromosome synXVI of the Sc2.0 project is described. Application of the CRISPR D-BUGS protocol identified defective loci, which were modified to improve sporulation and recover wild-type like growth when grown on glycerol as...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2025-05-07 | PXD058199 | Pride
Yeast 2.0 Scramble Synthetic genome Yeast 
TranscriptomicsGene expression response to copper toxicity between coastal and open ocean strains of marine Synechococcus
Marine cyanobacteria are thought to be the most sensitive of the phytoplankton groups to copper toxicity, yet little is known of the transcriptional response of marine Synechococcus to copper shock. Global transcriptional response to two levels of copper shock was assayed in both a coastal and an o...
ORGANISM(S): Synechococcus sp. CC9311 
2010-05-17 | E-GEOD-13910 | biostudies-arrayexpress
TranscriptomicsMicroarray analysis of phosphate regulation in Synechococcus sp. WH8102
Primary productivity of open ocean environments, such as those inhabited by marine picocyanobacteria Synechococcus sp.WH8102, are often limited by low inorganic phosphate (P). To observe how this organism copes with P starvation, we constructed a full genome microarray and examined differences in ge...
ORGANISM(S): Synechococcus sp. WH 8102 
2010-05-18 | E-GEOD-13170 | biostudies-arrayexpress
TranscriptomicsPtrA is required for coordinate regulation of gene expression during phosphate depletion in a marine Synechococcus
The genome of the marine Synechococcus sp. WH8102 displays a minimal regulatory network yet physiological and molecular responses of this organism are tuned to episodic limitation for nitrogen and phosphorus. Microarray analyses have demonstrated a key role for the two-component regulatory system, P...
ORGANISM(S): Synechococcus sp. WH 8102 
2010-04-08 | E-GEOD-18511 | biostudies-arrayexpress
TranscriptomicsD. nodosus grown in the presence/absence of ground hoof
This study aimed to identify genes that were differentially expressed when D. nodosus was grown on agar media supplemented with ground hoof compared to the standard agar media. Bacteria were grown on EYE agar or EYE agar with 2% ground hoof agar plates for 2 days before Trizol extraction of RNA. Fou...
ORGANISM(S): Dichelobacter nodosus 
2010-07-01 | E-GEOD-5166 | biostudies-arrayexpress
TranscriptomicsJIR1201, JIR1212, JIR1244, JIR1461, JIR1474, JIR3209, JIR3350, JIR3356 and JIR3528 genomic arrays
Comparing the genetic content of strains JIR1201, JIR1212, JIR1244, JIR1461, JIR1474, JIR3209, JIR3350, JIR3356 and JIR3528 to wild-type strain JIR3356. Comparison of JIR3356 with itself to eliminate any variation that may occur with the technique. This experiment should not produce any statistical...
ORGANISM(S): Dichelobacter nodosus 
2010-07-01 | E-GEOD-5157 | biostudies-arrayexpress
TranscriptomicsTranscriptomic response to substrate and temperature in two thermophilic fungi, Myceliophthora thermophila and Thielavia terrestris, and a related mesophile, Chaetomium globosum
The thermophilic filamentous fungi Myceliophthora thermophila (Sporotrichum thermophile) and Thielavia terrestris are proficient decomposers of cellulose, suggesting that they will be a rich source of thermostable industrial enzymes for lignocellulose degradation. To identify the genes and proteins...
ORGANISM(S): Myceliophthora thermophila 
2011-08-08 | E-GEOD-27323 | biostudies-arrayexpress
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