Transcriptional profiling of H9 hESCs (wild type and BRACHYURY shRNA knockdown) differentiated for 36h or 72h in chemically-defined culture media supplemented with FGF2, LY294002 and either BMP4 or ActivinA.
Comparison of H9 human embryonic stem cells, grown in chemically defined and animal-product free conditions, with their differentiated progeny - mesendodermal progenitors arisen as the result of changes made to chemically defined hESC growth conditions. Contributes to a larger work on the use of che...
Human Embryonic Stem Cells (hESC) grown in chemically defined medium (CDM) supplemented with Activin and FGF were compared to hESCs grown for 48 hours in CDM supplemented with FGF2 and SB431542, an inhibitor of Activin/Nodal signalling. The objective of this experiment was to discover the identity o...
