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ProteomicsProteome profiling of HaCaT keratinocytes
An important step in the proteomic analysis is the optimal extraction and the processing of protein material in order to ensure the highest sensitivity in downstream protein detection. Cell lysis is important for proteomic profiling with an eventual aim to discover differently expressed proteins (DE...
ORGANISM(S): Homo sapiens (Human) 
2022-07-20 | PXD035202 | Pride
Lc-ms/ms  searchgui  double-strand break repair via break-induced replication pathway  mcm family Hacat keratinocytes  sodium dodecyl sulfate  1de-gel concentration  osmotic shock 
ProteomicsHuman chorionic villus proteome
We evaluated the effect of the different solubilization techniques on the reproducibility and width of the chorionic proteome characterized using bottom-up proteomics: SDS-based solubilization coupled with in-gel protein digestion after applying self-named 1DE gel-concentration procedure (SDS-PAGE w...
ORGANISM(S): Homo sapiens (Human) 
2022-06-17 | PXD024759 | Pride
Human Chorionic villus Trophoblast Lc-ms/ms 
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