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ProteomicsProteomic Profiling of HEK293-F cell and human peripheral blood neutrophil proteins extracted by conventional direct extraction and phenol-guanidinium isothiocyanate (P/GTC)-based extraction methods
To evaluate the performance of the phenol-guanidinium isothiocyanate (P/GTC)-based extraction method in proteomic analysis, we performed proteome analysis from HEK293-F cell and human peripheral blood neutrophil proteins extracted by the P/GTC-based extraction and conventional direct extraction meth...
ORGANISM(S): Homo Sapiens (human) 
2019-10-28 | PXD011487 | JPOST Repository
ProteomicsFeasibility of phosphoproteomics on left-over sample after RNA extraction (RNA-Bee)
In daily practice biomolecules are usually extracted with their own specific protocols for downstream omics analysis. However, when sample material is limited, an all-in-one strategy is preferable. DNA, RNA and proteins can be isolated with phenol guanidine thiocyanate based extraction, yet lysis wi...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-04-22 | PXD023665 | Pride
Phenol guanidine thiocyanate Tissue Lysis Rnab Phosphoproteomics Cell line 
TranscriptomicsRNA-seq of human patient-derived xenograft pancreatic cancer Panc185 sphere-derived cells with the ISG15 gene edited using CRISPR-CAS9 against control spheres.
The goal of the study was to identify genes and pathways that were altered in the absence of the gene Interferon-Stimulated Gene 16 (ISG15) in human pancreatic ductal adenocarcinoma (PDAC) cancer stem cells. Primary adherent cultures established from patient-derived xenograft passaged in mice were e...
ORGANISM(S): Homo sapiens 
2020-04-30 | E-MTAB-8984 | biostudies-arrayexpress
ProteomicsNorthern elephant seal outer blubber layer proteome
Proteins were extracted from the outer (closer to skin) blubber layer of 4 northern elephant seals (Mirounga angustirostris) using either 1) A standard detergent lysis buffer containing 1% sodium deoxycholate, 8 M urea, 5 mM DTT in 50 mM ammonium bicarbonate (SDC method) or 2) the phenol and guanidi...
ORGANISM(S): Mirounga Angustirostris (ncbitaxon:9716) 
2018-06-12 | MSV000082455 | MassIVE
TranscriptomicsRNA-seq of human patient-derived xenograft pancreatic cancer PancA6L cells cultured for 14 days with Galactose versus PancA6L cells cultured for 14 days with Glucose
The goal of the study was to identify genes and pathways that were altered when human pancreatic ductal adenocarcinoma (PDAC) cancer cells are cultured with different carbon source (Glucose versus Galactose). Primary adherent cultures established from patient-derived xenograft passaged in mice were...
ORGANISM(S): Homo sapiens 
2020-09-01 | E-MTAB-9483 | biostudies-arrayexpress
UnknownLow-cost RNA extraction method for highly scalable transcriptome studies.
Not available
S-EPMC7495201 | biostudies-literature
TranscriptomicsIdentification of genes that may predict response to azacitidine treatment.
Azacitidine (AZA) is a hypomethylating drug used to treat disorders associated with myelodysplasia and related neoplasms. Approximately 50% of patients do not respond to AZA and have very poor outcomes. It is of great interest to identify predictive biomarkers for AZA responsiveness. Therefore, we s...
ORGANISM(S): Homo sapiens 
2016-02-10 | E-GEOD-77750 | biostudies-arrayexpress
Transcriptomicsimpact of polyphenols on miRNA expression in liver
At eight weeks of age, mice were divided into 10 groups with six mice per group and fed either the control-diet or the same diet supplemented with quercetin, hesperidin, naringenin, anthocyanin, catechin and curcumin at 0.006% (w/w) corresponding to an equivalent intake in humans of 30 mg/day, while...
ORGANISM(S): Mus musculus 
2012-04-13 | E-MEXP-3204 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human leukocytes from chronic myeloid leukemia patients exhibiting differing responses to imatinib
This is a class prediction experiment, where the class is the response status to imatinib (also called Gleevec), a drug used to treat patients with chronic myelogenous leukemia (CML). There are two data sets, a training set (from Leipzig, 8 Responders and 5 Non-Responders) and a validation set (fro...
ORGANISM(S): Homo sapiens 
2007-08-25 | E-GEOD-2535 | biostudies-arrayexpress
UnknownA sample preparation workflow for adipose tissue shotgun proteomics and proteogenomics.
Not available
S-EPMC6262853 | biostudies-literature
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