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TranscriptomicsRNA-seq of coding RNA of Pichia pastoris GS115 to determine the effect of inducing heterologous expression of human lysozyme (HuLy) variants
To determine the effect of inducing heterologous expression of human lysozyme (HuLy) variants in Pichia pastoris
ORGANISM(S): Pichia pastoris 
2013-09-12 | E-MTAB-1126 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Pichia pastoris treated with dithiothereitol to induce unfolded protein response
Experiment aims to induce unfolded protein response in Pichia pastoris, set-up was chosen to be comparable results to DTT experiment.
ORGANISM(S): Pichia pastoris 
2008-09-23 | E-MEXP-1615 | biostudies-arrayexpress
TranscriptomicsCatabolite repression of phosphoenolpyruvate carboxykinase by a zinc finger protein under biotin- and pyruvate carboxylase-deficient conditions in Pichia pastoris
We have identified a methanol- and biotin-starvation-inducible zinc finger protein named ROP [repressor of phosphoenolpyruvate carboxykinase (PEPCK)] in the methylotrophic yeast Pichia pastoris. When P.pastoris strain GS115 (wild-type, WT) is cultured in biotin-deficient, glucose ammonium (Bio-) ...
ORGANISM(S): Pichia pastoris 
2011-10-13 | E-GEOD-32948 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Pichia pastoris wild type vs recombinant Fab expressing strain with different osmolarities
Pichia pastoris was grown in carbon limited chemostat cultures on mineral medium with glucose as carbon source. 3 different osmolarities were selected (140, 850, 1400mOsmol kg-1). Samples were taken at steady-state, thus the effect of osmolarity in adapted cells was monitored.
ORGANISM(S): Pichia pastoris 
2010-10-26 | E-MEXP-2433 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of Pichia pastoris transformed with a vector carrying Fab3H6 and grown in different oxygen levels
The effect of oxygen availability on recombinant protein production in Pichia pastoris
ORGANISM(S): Pichia pastoris 
2010-06-09 | E-MEXP-2742 | biostudies-arrayexpress
TranscriptomicsComparison of gene expression profile among Pichia pastoris strain with a different gene dosage
Pichia pastoris is a widely used yeast platform for heterologous protein production. Although increasing gene dosage is a powerful strategy to improve recombinant protein production, an excess in the number of gene copies often leads to decreased product yields and increased metabolic burden. In ord...
ORGANISM(S): Komagataella pastoris 
2017-02-22 | E-MTAB-4582 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human dermal enothelial cells treated with 200ng/ml human recombinant endostatin (Pichia Pastoris)
Cells were treated with 200ng/ml human recombinant endostatin (Pichia Pastoris) for 6h at 37°C with 5 % CO2 and 95 % humidity.
ORGANISM(S): Homo sapiens 
2013-01-30 | E-RZPD-10 | biostudies-arrayexpress
TranscriptomicsPichia pastoris Glucose/Methanol comparison
Comparison of transcription profile of Pichia pastoris cells grown on Glucose medium with Pichia pastoris cells grown on Methanol/Glycerol medium, the fermentations were done in a chemostat. 2 color experiment in reference design. Pichia pastoris reference mix [mixed pool of Pichia pastoris cells sa...
ORGANISM(S): Komagataella pastoris CBS 7435 
2015-08-31 | E-GEOD-67690 | biostudies-arrayexpress
ProteomicsProteomic analysis of Pichia pastoris GS115
Pichia pastoris GS115 was cultured in YPD medium and protein were extracted and separated on SDS-PAGE followed by in-gel trypsin digestion. Protein were also digested in-solution using trypsin and fractionated using SCX and bRPLC. A total of 101 peptide fractions were analyzed on LTQ-Orbitrap Velos ...
ORGANISM(S): Komagataella pastoris (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris) 
2014-11-18 | PXD000627 | Pride
Pichia Lc-ms/ms 
TranscriptomicsTranscription profiling of Pichia pastoris strain GS115 transformed with the empty vector pGAPHIS or consitutively overproducing the activated form of S. cerevisiae Hac1
Strain GS115 transformed with the empty vector pGAPHIS (a histidine prototrophic isogenic strain of GS115) was compared to Strain GS115 HAC1, constitutively overproducing the activated form of S. cerevisiae Hac1
ORGANISM(S): Pichia pastoris 
2008-09-23 | E-MEXP-1616 | biostudies-arrayexpress
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