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ProteomicsGlobal proteomic alterations in extracellular vesicles induced by oncogenic PIK3CA mutations
MCF10A cells, and two isogenic mutant cell lines with known mutations in PIK3CA (E545 K and H1047R) were cultured in triplicate using culture medium depleted of extracellular vesicles. Cell culture supernatants were collected to study the extracellular vesicles (EVs) released by these cells. EVs wer...
ORGANISM(S): Homo sapiens (Human) 
2023-10-22 | PXD031718 | Pride
Mcf10a cells Extracellular vesicles Proteomics Pik3ca 
TranscriptomicsReactivation of multipotency by oncogenic PIK3CA induces breast tumor heterogeneity
Breast cancer is the most frequent cancer in women and consists of heterogeneous types of tumours that are classified into different histological and molecular subtypes1-3. Pik3ca and p53 are the two most frequently mutated genes and are associated with different types of human breast cancers4. The ...
ORGANISM(S): Mus musculus 
2015-08-13 | E-GEOD-69290 | biostudies-arrayexpress
ProteomicsHigh-dose oncogenic PIK3CA drives constitutive cellular stemness through increased NODAL/TGFb signaling
Oncogenic PIK3CA mutations activate phosphoinositide 3-kinase (PI3K) and are among the commonest somatic mutations in cancer and mosaic, developmental overgrowth disorders. We recently demonstrated that the ‘hotspot’ variant PIK3CAH1047R exerts striking allele dose-dependent effects on stemness in h...
ORGANISM(S): Homo sapiens (Human) 
2021-03-03 | PXD014719 | Pride
Proteomics Tfgb Nodal ipsc Cancer Transcriptomics Pik3ca 
ProteomicsActivating Mutations in PIK3CA result in Widespread Modulation of the Tyrosine Phosphoproteome
The human oncogene PIK3CA is frequently mutated in human cancers. The two hotspot mutations in PIK3CA, E545K and H1047R, have been shown to regulate widespread signaling events downstream of AKT. However, the impact of these activating mutations on the tyrosine phosphorylation signaling in the cell ...
ORGANISM(S): Homo sapiens (Human) 
2018-10-24 | PXD001460 | Pride
Human Breast cancer Phosphoproteomics Pik3ca Tyrosine 
TranscriptomicsTranscription profiling by array of human estrogen receptor alpha positive breast tumors with and without PIK3CA mutations.
PI3K/AKT pathway plays one of pivotal roles in breast cancer development and maintenance. PIK3CA, coding PIK3 catalytic subunit, is the oncogene which shows the high frequency of gain-of-function mutations leading to the PI3K/AKT pathway activation in breast cancer. In particular in the ERα-positive...
ORGANISM(S): Homo sapiens 
2011-01-10 | E-GEOD-22035 | biostudies-arrayexpress
TranscriptomicsGene expression profiling of preneoplastic K8-creERT2/PIK3CAH1047R mammary subsets
This study examined the effect of mutant PIK3CAH1047R expression in mammary subsets of preneoplastic mammary glands from K8-creERT2/PIK3CA H1047R mice Mammary cell subpopulations were isolated from K8-creERT2/PIK3CAH1047R and K8-creERT2 control animals 4 weeks after activation of PIK3CA H1047R trans...
ORGANISM(S): Mus musculus 
2015-08-12 | E-GEOD-65411 | biostudies-arrayexpress
ProteomicsPhosphoproteomic analysis of breast epithelial cells with isogenic knockin of PIK3CA mutations
The PIK3CA gene is frequently mutated in human cancers. To study the signaling mechanisms responsible for cell growth and invasion phenotypes induced by mutant PIK3CA molecules, we carried out a SILAC-based quantitative phosphoproteomic analysis of MCF10A, a spontaneously immortalized normal mammary...
ORGANISM(S): Homo sapiens (Human) 
2015-07-28 | PXD000599 | Pride
Breast cancer Ls-ms/ms Pik3ca Phosphorylation Proteome Mutation 
GenomicsExpression data PIK3CA E545K vs PIK3CA H1047R stably transfected in human HCC cell lines
Expression data PIK3CA E545K vs PIK3CA H1047R stably transfected in human HCC cell lines
PRJNA758291 | ENA
TranscriptomicsConditional loss of ErbB3 delays mammary gland hyperplasias induced by mutant PIK3CA but does not affect mammary tumor latency, gene expression or signaling
Mutations in PIK3CA, the gene encoding the p110α subunit of PI3K, induce transformation of mammary epithelial cells (MEC). Studies suggest the transforming action of mutant PIK3CA requires binding to activated receptor tyrosine kinases or adaptors. We examined in transgenic mice if ErbB3, a powerful...
ORGANISM(S): Mus musculus 
2013-06-20 | E-GEOD-43037 | biostudies-arrayexpress
GenomicsRNA sequencing of PIK3CA-WT/WT and PIK3CA-WT/H1047R primary mouse embryonic fibroblasts
RNA sequencing of PIK3CA-WT/WT and PIK3CA-WT/H1047R primary mouse embryonic fibroblasts
PRJNA557222 | ENA
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