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ProteomicsGlobal ubiquitylation analysis of mitochondria in primary neurons following PINK1-dependent activation of Parkin
Dataset analyzed in "Global ubiquitylation analysis of mitochondria in primary neurons following PINK1-dependent activation of Parkin" paper. Experiment TMT-labeled neurons untreated vs 5h AO treatment: 1) Membrane Proteome: .raw files AO2445-AO2456 2) Ubiquitylome: .raw files AO2433-AO2438 Experi...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2021-06-16 | MSV000087639 | MassIVE
TranscriptomicsParkinson Phenotype in Aged PINK1-Deficient Mice Is Accompanied by Progressive Mitochondrial Dysfunction in Absence of Neurodegeneration
Parkinson's disease (PD) is an adult-onset movement disorder of largely unknown etiology. We have previously shown that loss-of-function mutations of the mitochondrial protein kinase PINK1 (PTEN induced putative kinase 1) cause the recessive PARK6 variant of PD. Now we generated a PINK1 deficient mo...
ORGANISM(S): Mus musculus 
2014-11-04 | E-GEOD-60413 | biostudies-arrayexpress
TranscriptomicsPotentiation of neurotoxicity in double mutant mice with Pink1 ablation and A53T-SNCA overexpression
As the second most frequent neurodegenerative disorder of old age, ParkinsonM-bM-^@M-^Ys disease (PD) can result from autosomal dominant causes like increased alpha-synuclein (SNCA) dosage, or from autosomal recessive causes like PINK1 loss-of-function. Interactions between these triggers and their ...
ORGANISM(S): Mus musculus 
2014-11-04 | E-GEOD-60414 | biostudies-arrayexpress
ProteomicsAbsolute quantification of peptides from TIM23 or PINK1-3HA using AQUA peptides
To quantify TIM23 and PINK1-3HA in the immunoprecipitates, control or PINK1-3HA expressing HeLa cells were treated with CCCP and subjected to immunoprecipitation with anti-HA antibody bound to magnetic beads. After spiked with 200 fmol stable isotope-labeled AQUA peptides (Cosmo Bio Co Ltd) as stand...
ORGANISM(S): Homo Sapiens (human) 
2023-04-07 | PXD041244 | JPOST Repository
TranscriptomicsTranscriptional profile of pink1 mutant flies
Global analysis of transcriptional changes in Drosophila pink1 mutants.
ORGANISM(S): Drosophila melanogaster 
2013-12-02 | E-MEXP-3645 | biostudies-arrayexpress
ProteomicsPINK1-TOM-TIM23 complex SDA XL-MS
3xFLAG-tagged human wild-type PINK1 was expressed and immunoprecipitated with anti-FLAG beads. Chemical cross-linking MS (XL-MS) analysis using SDA and UV exposure was performed followed by in solution trypsin digestion and MS analysis.
ORGANISM(S): Homo Sapiens (human) 
2025-05-29 | PXD058526 | JPOST Repository
ProteomicsPhosphoproteome of PINK1 in HEK293 cells
triple SILAC phosphoproteomics experiment of Flp-In T-Rex HEK293 cells stably expressing either FLAG empty, PINK1-FLAG Kinase dead or PINK1-FLAG wild type were grown in ‘light’ (K0R0), ‘medium’ (K4R6) and ‘heavy’ (K8,R10) SILAC media, respectively.
ORGANISM(S): Homo sapiens (Human) 
2015-09-16 | PXD002127 | Pride
Pink1 Parkinson's disease Human Phosphorylation Phosphoproteomics Hek293 
ProteomicsTom20 gates PINK1 activity and mediates its tethering of the TOM and TIM23 translocases upon mitochondrial stress
Mutations in PTEN-induced putative kinase 1 (PINK1) cause autosomal recessive early onset Parkinson disease (PD). PINK1 is a Ser/Thr kinase that regulates mitochondrial quality control by triggering mitophagy mediated by the ubiquitin ligase Parkin. Upon mitochondrial damage, PINK1 accumulates on th...
ORGANISM(S): Homo sapiens (Human) 
2024-03-02 | PXD047765 | Pride
Pink1 Human Tom complex Mitochondria Tim complex Parkinson's 
ProteomicsLC-MS/MS analysis of co-immunoprecipitates with PINK1-3FLAG
Parental HeLa cells and HeLa cells stably expressing PINK1-3FLAG were treated with or without 10 μM CCCP for 3 h, incubated with 0.1% paraformaldehyde for 10 min at room temperature, and then quenched with 100 mM glycine-NaOH (pH7.5) for 4 min. After washing twice with 20 mM HEPES-NaOH (pH7.4) and 1...
ORGANISM(S): Homo Sapiens (human) 
2023-04-07 | PXD041241 | JPOST Repository
ProteomicsStructure of human PINK1 at a mitochondrial TOM/VDAC array
We report here the structure of human PINK1 stabilized mid-import at an array of endogenous TOM and VDAC complexes purified from human mitochondria. In our structure, two TOM core complexes are separated by a dimer of VDAC2 channels, and an inactive PINK1 dimer is inserted into the VDAC-proximal TOM...
ORGANISM(S): Homo sapiens (Human) 
2025-05-05 | PXD058529 | Pride
Pink1 Cryoem Xl-ms Parkinson's diseease 
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