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TranscriptomicsWhole transcriptome profiling of successful immune response to Vibrio infections in the oyster Crassostrea gigas by digital gene expression analysis
The cultivated Pacific oyster Crassostrea gigas has suffered for decades large scale summer mortality phenomenon resulting from the interaction between the environment parameters, the oyster physiological and/or genetic status and the presence of pathogenic microorganisms including Vibrio species. T...
ORGANISM(S): Crassostrea gigas 
2011-11-01 | E-GEOD-33390 | biostudies-arrayexpress
TranscriptomicsInduction of a peptide with activity against a broad spectrum of pathogens in the Aedes aegypti salivary gland, following infection with dengue virus
Dengue viruses (DENV) are generally maintained in a cycle which requires horizontal transmission via their arthropod vector, Ae. Aegypti, to the vertebrate host. One important consequence of this process is the interference of these arboviruses with both invertebrate and vertebrate immune systems. W...
ORGANISM(S): Aedes aegypti 
2011-03-21 | E-GEOD-21528 | biostudies-arrayexpress
TranscriptomicsTranscriptome analysis of monocytic leukemia cell differentiation
The human leukemia cell line U937 is a well-established model for studying monocytic cell differentiation. We used a modified protocol (SADE) of serial analysis of gene expression (SAGE) and developed a SADE linker-anchored PCR assay to investigate the pattern of expression of known genes and to ide...
ORGANISM(S): Homo sapiens 
2004-10-23 | E-GEOD-1865 | biostudies-arrayexpress
TranscriptomicsBlood mRNA profile during EPO treatment
In the present study, we obtained a global view from the transcriptome of total blood cells with or without darbepoetin alpha -an Erythropoiesis-Stimulating Agent (ESAs)- treatment using the SAGE method, which allows large-scale quantitative gene expression measurements. We sequenced 60,000 tags fr...
ORGANISM(S): Homo sapiens 
2009-12-31 | E-GEOD-15590 | biostudies-arrayexpress
TranscriptomicsSimultaneous gene expression profiling in human macrophages infected with Leishmania major parasites using SAGE
Leishmania (L) are intracellular protozoan parasites which are able to survive and replicate within the harsh and potentially hostile phago-lysosomal environment of mammalian mononuclear phagocytes. A complex interplay then takes place between the macrophage (MM-NM-&) striving to eliminate the patho...
ORGANISM(S): Leishmania major 
2008-05-21 | E-GEOD-10442 | biostudies-arrayexpress
TranscriptomicsAtlas of gene expression in the mouse kidney
To gain molecular insight into kidney function, we performed a high-resolution quantitative analysis of gene expression in glomeruli and nine different nephron seg-ments dissected from mouse kidney using the long-SAGE method. We also developed dedicated bioinformatics tools and databases to annotate...
ORGANISM(S): Mus musculus 
2010-11-11 | E-GEOD-25223 | biostudies-arrayexpress
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