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TranscriptomicsUAP56 couples piRNA clusters to the perinuclear transposon silencing machinery [tiling array]
The transposon silencing piRNAs are produced from precursors that are encoded by heterochromatic clusters and processed in the perinuclear nuage. We show that the Drosophila nuclear DEAD box protein UAP56, previously implicated in mRNA splicing and nuclear export, co-localizes with the cluster-assoc...
ORGANISM(S): Drosophila melanogaster 
2012-11-28 | E-GEOD-35636 | biostudies-arrayexpress
TranscriptomicsUAP56 couples piRNA clusters to the perinuclear transposon silencing machinery [RNA-Seq and RIP-Seq]
The transposon silencing piRNAs are produced from precursors that are encoded by heterochromatic clusters and processed in the perinuclear nuage. We show that the Drosophila nuclear DEAD box protein UAP56, previously implicated in mRNA splicing and nuclear export, co-localizes with the cluster-assoc...
ORGANISM(S): Drosophila melanogaster 
2012-11-28 | E-GEOD-35637 | biostudies-arrayexpress
TranscriptomicsAlkbh1/Tzfp Repress a Non-Repeat piRNA Cluster in Pachytene Spermatocytes
Piwi proteins and Piwi-interacting small RNAs (piRNAs) have known functions in transposon silencing in the male germline of fetal and newborn mice. Both are also necessary for spermatogenesis in adult testes, however, their function here remains a mystery. Here, we use germ cell isolations and small...
ORGANISM(S): Mus musculus 
2012-09-11 | E-GEOD-37150 | biostudies-arrayexpress
TranscriptomicsHigh throughput sequencing of Piwi bound piRNAs from Drosophila ovaries in which key factors for primary piRNA biogenesis in somatic support cells were knocked down using RNAi
In Drosophila, PIWI proteins and bound PIWI interacting RNAs (piRNAs) form the core of a small RNA mediated defense system against selfish genetic elements. Within germline cells piRNAs are processed from piRNA clusters and transposons to be loaded into Piwi/Aubergine/AGO3 and a subset of piRNAs und...
ORGANISM(S): Drosophila melanogaster 
2010-10-03 | E-GEOD-23560 | biostudies-arrayexpress
TranscriptomicsThe Rhino-Deadlock-Cutoff complex licenses non-canonical transcription of dual-strand piRNA clusters in Drosophila
Argonaute proteins of the PIWI clade are central to transposon silencing in animal gonads. Their target specificity is defined by 22-30nt PIWI interacting RNAs (piRNAs), which mostly originate from discrete genomic loci termed piRNA clusters. Here we show that the RDC complex composed of Rhino, Dead...
ORGANISM(S): Drosophila melanogaster 
2014-06-08 | E-GEOD-55824 | biostudies-arrayexpress
TranscriptomicspiRNA production requires heterochromatin formation in Drosophila
Here, we analyzed two small RNA libraries derived from ovarian tissue mutant for either the Drosophila SETDB1 gene, or the Bam gene. Here we show that deposition of histone 3 lysine 9 by the methyltransferase dSETDB1 (egg) is required for piRNA cluster transcription. In the absence of dSETDB1, clust...
ORGANISM(S): Drosophila melanogaster 
2011-08-15 | E-GEOD-30086 | biostudies-arrayexpress
TranscriptomicsThe Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline
In a broad range of organisms, Piwi-interacting RNAs (piRNAs) have emerged as core components of a surveillance system that protects the genome by silencing transposable and repetitive elements. A vast proportion of piRNAs is produced from discrete genomic loci, termed piRNA clusters. The molecular ...
ORGANISM(S): Drosophila melanogaster 
2013-06-07 | E-GEOD-47738 | biostudies-arrayexpress
TranscriptomicsSpecialized piRNA pathways act in germline and somatic tissues of the Drosophila ovary
Here, we analyzed nine Drosophila piRNA pathway mutants for their impacts on both small RNA populations and the sub-cellular localization patterns of Piwi proteins. We find that distinct piRNA pathways with differing components function in ovarian germ and somatic cells. Keywords: Epigenetics 20 li...
ORGANISM(S): Drosophila melanogaster 
2009-04-29 | E-GEOD-15186 | biostudies-arrayexpress
TranscriptomicspiRNA-guided slicing of transposon transcripts enforces their transcriptional silencing via specifying the nuclear piRNA repertoire
PIWI-clade Argonaute proteins silence transposon expression in animal gonads. Their target specificity is defined by bound ~23-30nt piRNAs that are processed from single-stranded precursor transcripts via two distinct pathways. Primary piRNAs are defined by the endo-nuclease Zucchini, while biogenes...
ORGANISM(S): Drosophila melanogaster 
2015-08-31 | E-GEOD-71775 | biostudies-arrayexpress
GenomicspiRNA production requires heterochromatin formation in Drosophila
Here, we analyzed H3K9me3 derived from wild type ovarian tissue. Here we show that deposition of histone 3 lysine 9 by the methyltransferase dSETDB1 (egg) is required for piRNA cluster transcription. In the absence of dSETDB1, cluster precursor transcription collapses in germline and somatic gonadal...
ORGANISM(S): Drosophila melanogaster 
2011-09-08 | E-GEOD-30157 | biostudies-arrayexpress
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