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In this study, the native Sinapis alba plastid-encoded RNA polymerase (PEP) complex was purified and cross-linking MS was used to help in its structure determination.
ORGANISM(S): Sinapis alba 
2024-03-01 | PXD045575 | Pride
RNA polymerases (RNAPs) transcribe DNA into RNA and are found in all living organisms with several degrees of complexity from single polypeptide chain to multimeric enzymes. In chloroplast of angiosperms, two RNAPs are involved in plastid gene transcription: the nuclear-encoded RNA polymerase (NEP) ...
ORGANISM(S): Sinapis alba 
2022-09-12 | PXD032738 | Pride
RNA polymerases (RNAPs) transcribe DNA into RNA and are found in all living organisms with several degrees of complexity from single polypeptide chain to multimeric enzymes. In chloroplast of angiosperms, two RNAPs are involved in plastid gene transcription: the nuclear-encoded RNA polymerase (NEP) ...
ORGANISM(S): Sinapis alba 
2022-09-12 | PXD032739 | Pride
Gene expression in plastids of higher plants is dependent on two different transcription machineries, a plastid-encoded bacterial-type RNA polymerase (PEP) and a nuclear-encoded phage-type RNA polymerase (NEP), which recognize distinct types of promoters. The division of labor between PEP and NEP du...
ORGANISM(S): Hordeum vulgare 
Retrograde signaling from the chloroplast to the nucleus is necessary to regulate the chloroplast proteome during development and fluctuating environmental conditions. Although the specific chloroplast process(es) that must occur and the nature of the signal(s) that exits the chloroplast are not wel...
ORGANISM(S): Arabidopsis thaliana 
Genome-wide binding patterns of the Plastid-encoded polymerase in Arabidopsis
pTAC3 and pTAC14 are required for binding of Plastid Encoded RNA Polymerase to DNA
Chloroplasts retain a complex Plastid-Encoded RNA polymerase (PEP) despite having drastically reduced genomes compared to their cyanobacterial ancestors. In land plants, PEP comprises a bacterial-like catalytic core and 15 nuclear-encoded accessory subunits, whose evolutionary origins and functions ...
ORGANISM(S): Chlamydomonas reinhardtii 
2026-09-30 | PXD073490 | Pride
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