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TranscriptomicsRNA-seq analysis of HeLa cells treated with GapmeR antisense oligonucleotides
The experiment was carried out to provide insights into biological function of a newly identified long noncoding RNA, PNCTR. HeLa cells were transfected with either a PNCTR-specific GapmeR antisense oligonucleotide or a non-targeting GapmeR control. Total RNAs were extracted at 24 hours post transfe...
ORGANISM(S): Homo sapiens 
2018-10-12 | E-MTAB-6529 | biostudies-arrayexpress
ProteomicsLabel-free LC-MS/MS analysis of proteins associated with specific RNAs in HeLa cells
Proteins co-localizing with RNA targets of interest in HeLa cells were identified using our recently optimized hybridization-proximity labeling approach (HyPro2) combined with label-free mass spectrometry. Briefly, fixed and permeabilized cells were hybridized with digoxigenin-labeled antisense olig...
ORGANISM(S): Homo sapiens (Human) 
2025-08-04 | PXD063191 | Pride
Actb Rna-protein interactions Lncrna Fus Pre-mrna Hybridization-proximity labeling Pnctr. 
TranscriptomicsHyPro-seq analysis of HeLa and ARPE-19 cells
The ability of RNAs to form specific contacts with other macromolecules provides an important mechanism for subcellular compartmentalization. We developed a suite of hybridization-proximity (HyPro) labeling technologies for unbiased discovery of proteins (HyPro-MS) and transcripts (HyPro-seq) associ...
ORGANISM(S): Homo sapiens 
2021-11-05 | E-MTAB-10365 | biostudies-arrayexpress
UnknownA Short Tandem Repeat-Enriched RNA Assembles a Nuclear Compartment to Control Alternative Splicing and Promote Cell Survival.
Not available
S-EPMC6224606 | biostudies-literature
GenomicsRNA-seq analysis of HeLa cells treated with GapmeR antisense oligonucleotides
RNA-seq analysis of HeLa cells treated with GapmeR antisense oligonucleotides
PRJEB25110 | ENA
ProteomicsHyPro-MS analysis of proteins proximal to nuclear noncoding RNAs in HeLa cells
The ability of RNAs to form specific contacts with other macromolecules provides an important mechanism for subcellular compartmentalization. We developed a suite of hybridization-proximity (HyPro) labeling technologies for unbiased discovery of proteins (HyPro-MS) and transcripts (HyPro-seq) associ...
ORGANISM(S): Homo sapiens (Human) 
2021-11-08 | PXD025264 | Pride
Human Hela cells Orbitrap fusion lumos tribrid Lc-ms/ms 
UnknownHybridization-proximity labeling reveals spatially ordered interactions of nuclear RNA compartments.
Not available
S-EPMC8791277 | biostudies-literature
GenomicsHyPro-seq analysis of HeLa and ARPE-19 cells
HyPro-seq analysis of HeLa and ARPE-19 cells
PRJEB44394 | ENA
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