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GenomicsHog1 controls global reallocation of RNA Pol II upon osmotic shock
When challenged with osmotic shock, S. cerevisiae induces hundreds of genes, despite a global reduction in transcriptional capacity. The mechanisms that regulate this rapid reallocation of transcriptional resources are not known. Here we show that redistribution of RNA Pol II upon stress requires ...
ORGANISM(S): Saccharomyces cerevisiae 
2012-07-15 | E-GEOD-38208 | biostudies-arrayexpress
TranscriptomicsProfiling of RNA Pol II occupancy in Drosophila neural stem cell populations using TaDa (Targeted DamID)
Cell-type specific transcriptional profiling is key to understanding cell fate specification and function. In order to achieve this it has been necessary, to date, to isolate specific cell types from complex tissues. We have developed 'TaDa', a technique that enables cell-specific profiling without ...
ORGANISM(S): Drosophila melanogaster 
2013-06-21 | E-GEOD-46803 | biostudies-arrayexpress
GenomicsPol II and its associated epigenetic marks are present at pol III-transcribed non-coding RNA genes (I)
Epigenetic control is an important aspect of gene regulation. Despite detailed understanding of many examples, the transcription of non-coding RNA genes by RNA polymerase (pol) III is less well characterized. Here we profile the epigenetic features of pol III target genes throughout the human gen...
ORGANISM(S): Homo sapiens 
2010-04-25 | E-GEOD-20040 | biostudies-arrayexpress
TranscriptomicsPol II and its associated epigenetic marks are present at pol III-transcribed non-coding RNA genes (II)
Epigenetic control is an important aspect of gene regulation. Despite detailed understanding of many examples, the transcription of non-coding RNA genes by RNA polymerase (pol) III is less well characterized. Here we profile the epigenetic features of pol III target genes throughout the human gen...
ORGANISM(S): Homo sapiens 
2010-04-25 | E-GEOD-20071 | biostudies-arrayexpress
GenomicsRegulation of Mediator-dependent transcriptional activation of Pol II(G) through TFII
Pol II(G) is a novel form of RNA polymerase II that contains a tightly associated Gdown1 polypeptide. Whereas Pol II suffices for robust activator-dependent transcription in the absence of Mediator, Pol II(G) is highly dependent upon Mediator in a biochemically defined in vitro system. However, the ...
ORGANISM(S): Homo sapiens 
2012-01-13 | E-GEOD-33128 | biostudies-arrayexpress
TranscriptomicsThe RNA Pol II Elongation Factor Ell3 Marks Enhancers in ES Cells and Primes Future Gene Activation
Enhancers play a central role in precisely regulating the spatiotemporal expression of developmentally regulated genes. The molecular mechanisms and factors required for controlling the inactive or poised enhancers and their function in future gene activation remain elusive. Here, we have identified...
ORGANISM(S): Mus musculus 
2012-12-30 | E-GEOD-38148 | biostudies-arrayexpress
TranscriptomicsPostrecruitment Regulation of RNA Pol II Directs Rapid Signaling Responses at the Promoters of Estrogen Target Genes
Under current models for signal-dependent transcription in eukaryotes, DNA-binding activator proteins regulate the recruitment of RNA polymerase II (Pol II) to a set of target promoters. Yet, recent studies, as well as our results herein, show that Pol II is widely distributed (i.e., "preloaded") a...
ORGANISM(S): Homo sapiens 
2010-05-14 | E-GEOD-13051 | biostudies-arrayexpress
TranscriptomicsRNA Pol III transcriptomes and relationships to Pol II promoters, enhancer-binding factors and chromatin domains
RNA polymerase III transcribes many noncoding RNAs (e.g. tRNAs) important for translational capacity and other functions. Here, we localized RNA polymerase III, alternative TFIIIB complexes (BRF1/2) and TFIIIC in HeLa cells, determining the Pol III transcriptome, defining gene classes, and reveali...
ORGANISM(S): Homo sapiens 
2010-04-14 | E-GEOD-20609 | biostudies-arrayexpress
GenomicsGenome-wide mapping of RNA Pol II binding site (GM12878)
We report the results of chromatin immunoprecipitation following by high-thoughput sequencing (ChIP-Seq) using the GA II platform from Illumina for the human RNA Polymerase II in GM12878 cells. We have also generated a sequenced input DNA datasets for GM12878 cells. For data usage terms and condit...
ORGANISM(S): Homo sapiens 
2009-12-31 | E-GEOD-19550 | biostudies-arrayexpress
TranscriptomicsMitotic Transcriptional Clearance of Actively Engaged Pol II Through Transcriptional Elongation Control
Chromatin is highly condensed and transcriptionally repressed during mitosis. Although it is established that some general transcription factors are inactivated by phosphorylation at mitosis, many details of mitotic transcriptional repression and its underlying mechanisms are largely unknown. Here, ...
ORGANISM(S): Homo sapiens 
2015-10-30 | E-GEOD-71848 | biostudies-arrayexpress
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