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identification of proteins in polysome pellet with and without silvestrol treatment
ORGANISM(S): Homo sapiens (Human) 
2014-09-16 | PXD001310 | Pride
The goal was to measure the expression and polysome association of genes with polysomes in human embryonic stem cells H9 human embryonic stem cells were kept in pluripotent conditions. Total RNA and polysome extracts were isolated.
ORGANISM(S): Homo sapiens 
Translational control is a key regulatory step in the expression of genes as proteins. In plant cells, translational efficiency of mRNAs differs on different mRNA species, and the efficiency dynamically changes in various conditions. To gain a global view of translational control throughout growth a...
ORGANISM(S): Arabidopsis thaliana 
Polysome Nanopore Sequencing
Oocyte maturation, fertilization, and early embryonic development occur in the absence of gene transcription. Therefore, it is critical to understand at a global level the post-transcriptional events that are driving these transitions. Here, we have used a systems approach by combining polysome mRNA...
ORGANISM(S): Mus musculus 
Polysome-bound mRNA was analyzed to determine the effects of INK128 and radiation on mRNA translation 4 samples were analyzed with 3 biological replicates
ORGANISM(S): Homo sapiens 
The membrane fraction and the cytosolic fraction of HES2 cells were collected and subjected to microarray. The experiment was performed in triplicate Keywords: membrane-polysome fractionation of RNA Three consecutive passages of HES2 cells were grown and subject to membrane-polysome fractionation....
ORGANISM(S): Homo sapiens 
Gene expression in the total RNA and heavy polysome fractions of Eif4g3 siRNA treated lymph node stromal cells (LNSCs) compared to control-sIRNA treated samples The objective of this study was to identify genes whose translation are reduced after silencing Eif4g3 (the gene which encodes the translat...
ORGANISM(S): Mus musculus 
Arabidopsis polysome sequencing in nine tissues
Heat stress is one of the most prominent and deleterious environmental threads affecting plant growth and development. Upon high temperatures, plants launch specialized gene expression programs that promote stress protection and survival. These programs involve global and specific changes at the tra...
ORGANISM(S): Arabidopsis thaliana 
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