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MetabolomicsLipidomic analysis of lipid droplets from murine hepatocytes reveals distinct signatures for nutritional stress
Liver steatosis can be induced by fasting or high-fat diet. We investigated by lipidomic analysis whether such metabolic states are reflected in the lipidome of hepatocyte lipid droplets (LDs) from mice fed normal chow diet (FED), fasted (FAS), or fed a high-fat diet (HFD). LC-MS/MS at levels of lip...
2014-08-19 | MTBLS26 | MetaboLights
MetabolomicsEvidence of a DHA signature in the lipidome and metabolome of human hepatocytes
Cell supplementation with bioactive molecules often causes a perturbation in the whole intracellular environment. Omics techniques can be applied for the assessment of this perturbation. In this study, the overall effect of docosahexaenoic acid (DHA) supplementation on cultured human hepatocyte lipi...
2017-03-02 | MTBLS419 | MetaboLights
MetabolomicsUntargeted LC-MS metabolomics for the analysis of micro-scaled extracellular metabolites from hepatocytes
Metabolome analysis in micro physiological models is a challenge due to the low volume of cell culture medium (CCM). Here, we report a LC-MS-based untargeted metabolomics protocol for the detection of hepatocyte extracellular metabolites from micro-scale samples of CCM. Using a single LC-MS method w...
2021-01-04 | MTBLS1794 | MetaboLights
MetabolomicsGlobal Analyses of Selective Insulin Resistance in Hepatocytes due to Palmitate Lipotoxicity
Obesity is tightly linked to hepatic steatosis and insulin resistance. One feature of this association is the paradox of selective insulin resistance: insulin fails to suppress hepatic gluconeogenesis but activates lipid synthesis in the liver. How lipid accumulation interferes selectively with some...
2018-04-26 | MTBLS582 | MetaboLights
TranscriptomicsBenzo[a]pyrene response in primary mouse hepatocytes
Gene expression changes to several concentrations of benzo[a]pyrene in primary hepatocytes of wildtype and Xpa-/-p53+/- mice
ORGANISM(S): Mus Musculus 
2011-08-11 | E-TABM-1139 | biostudies-arrayexpress
TranscriptomicsDissecting modes of action of non-genotoxic carcinogens in primary mouse hepatocytes.
Under REACH, the European Community Regulation on chemicals, the testing strategy for carcinogenicity is generally based on in vitro and in vivo genotoxicity assays. Given that non-genotoxic carcinogens are negative for genotoxicity, this class of carcinogens will not be detected. Therefore, alterna...
ORGANISM(S): Mus musculus 
2012-06-25 | E-GEOD-35058 | biostudies-arrayexpress
ProteomicsMyristic acid induces proteomic changes in primary murine hepatocytes
Myristic acid, the 14-carbon saturated fatty acid (C14:0), is usually associated with negative consequences for human health, and in particular its consumption is correlated to an increased cardiovascular disease risk. Since it is a little abundant into the cells, its specific properties and functio...
ORGANISM(S): Mus musculus (Mouse) 
2022-03-01 | PXD009017 | Pride
Mouse Primary murine hepatocytes Myristic acid Lc-ms/ms 
TranscriptomicsAberrantly microRNA profiling in cancerous hepatocytes versus normal primary hepatocytes
MicroRNAs is a rapidly expanding area expected to change the way in which diseases will be diagnosed, treated and monitored in the future. Hepatocellular carcinoma (HCC) shows a rising incidence with high mortality but lack of effective targeted therapies. We identified the aberrantly expressed miRN...
ORGANISM(S): Homo sapiens 
2010-12-30 | E-GEOD-20077 | biostudies-arrayexpress
Transcriptomicsglobal gene expression profiles in primary human hepatocytes exposed to phytochemicals
Primary human hepatocytes were treated with 2 non-cytotoxic concentrations of sulforaphane (SFN; 10 and 50 uM) phenethyl isothiocyante (PEITC; 10 and 25 uM), 3,3'-diindolylmethane (DIM; 10 and 50 uM), or one concentration of oltipraz (OPZ; 30 uM) for 48 hours. Given the considerable heterogeneity o...
ORGANISM(S): Homo sapiens 
2010-07-19 | E-GEOD-20479 | biostudies-arrayexpress
ProteomicsFlucloxacillin-modified proteins identified in primary human hepatocytes
Flucloxacillin is a β-lactam antibiotic associated with a high incidence of drug-induced liver injury. Although expression of HLA-B*57:01 increases susceptibility, little is known of the pathological mechanisms involved in the induction of the clinical phenotype. Irreversible protein modification is...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD038581 | Pride
Primary human hepatocytes Flucloxaxillin Protein modification 
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