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ProteomicsProinsulin degradation and presentation of a proinsulin B-chain autoantigen involves ER-associated protein degradation (ERAD)-enzyme UBE2G2
Type 1 diabetes (T1D) is characterized by HLA class I-mediated presentation of autoantigens on the surface of pancreatic β-cells. Recognition of these autoantigens by CD8¬¬+ T cells results in the destruction of pancreatic β-cells and, consequently, insulin deficiency. Most epitopes presented at the...
ORGANISM(S): Homo sapiens (Human) 
2024-03-20 | PXD049996 | Pride
Ube2g2 Type 1 diabetes Insulin-derived peptides Proinsulin Hla-class i 
TranscriptomicsGene expression changes in response to doxycyline-induced expression of proinsulin (C96Y)-GFP in an INS-1 insulinoma cell line
A doxycyline-inducible INS-1 insulinoma cell line expressing proinsulin (C96Y)-GFP was engineered. Addition of doxycyline causes the production of the proinsulin (C96Y)-GFP, which is retained in the endoplasmic reticulum. This study analyzes the gene expression changes that occur after doxycyline-in...
ORGANISM(S): Rattus norvegicus 
2010-07-29 | E-GEOD-22537 | biostudies-arrayexpress
GenomicsIdentify proinsulin regulators using CRISPR screen and mouse genetics [RNA-Seq]
Identify proinsulin regulators using CRISPR screen and mouse genetics [RNA-Seq]
PRJNA1030055 | ENA
GenomicsIdentify proinsulin regulators using CRISPR screen and mouse genetics [CRISPR screen]
Identify proinsulin regulators using CRISPR screen and mouse genetics [CRISPR screen]
PRJNA1030057 | ENA
TranscriptomicsEye imaginal disc expressing transgenic human proinsulin in Drosophila eye under regulation of GMR-GAL4
We used microarrays to detail the global program of gene expression in response to expression of either mutant (C96Y) or wild-type human proinsulin and identified distinct classes of up-regulated genes. Results provides insight into the molecular mechanisms underlying a form of neonatal diabetes. Dr...
ORGANISM(S): Drosophila melanogaster 
2012-12-23 | E-GEOD-43128 | biostudies-arrayexpress
ProteomicsA reference map of the human proinsulin biosynthetic interaction network
A monoclonal antibody specific for human proinsulin (20G11) was generated and utilized for affinity purification mass spectrometry (AP-MS) for unbiased profiling of proinsulin protein:protein interactions in human islets. Half of each islet prep was treated with the cytokines IL1-beta and IFN-gamma....
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-07-03 | MSV000084057 | MassIVE
OtherIdentify proinsulin regulators using CRISPR screen and mouse genetics [CRISPR screen]
Proinsulin is the precursor of insulin in pancreatic beta cells. Altered proinsulin and proinsulin to insulin ratio mark beta cell dysfunction, predictuing disease progression into type 1 and type 2 diabetes. Of essential role for beta cell function, knowledge about proinsulin production and its rol...
ORGANISM(S): Mus musculus 
2023-11-15 | GSE245845 | GEO
TranscriptomicsIdentify proinsulin regulators using CRISPR screen and mouse genetics [RNA-Seq]
Proinsulin is the precursor of insulin in pancreatic beta cells. Altered proinsulin and proinsulin to insulin ratio mark beta cell dysfunction, predictuing disease progression into type 1 and type 2 diabetes. Of essential role for beta cell function, knowledge about proinsulin production and its rol...
ORGANISM(S): Homo sapiens Mus musculus 
2023-11-15 | GSE245844 | GEO
TranscriptomicsC-peptide regulates early transcription in rat proximal tubular cells
C-peptide exerts beneficial effects on glomerular hyperfiltration in type I diabetic patients. As C-peptide localizes to the nucleus, we investigated the transcriptional activities of C-peptide in proximal tubular cells isolated from diabetic rats. Two groups of proximal tubular cells isolated from ...
ORGANISM(S): Rattus norvegicus 
2011-07-26 | E-GEOD-24687 | biostudies-arrayexpress
GenomicsPancreatic β cell SERCA2 deficiency leads to impaired proinsulin processing and reduced ER to Golgi protein trafficking
Pancreatic β cell SERCA2 deficiency leads to impaired proinsulin processing and reduced ER to Golgi protein trafficking
PRJNA855926 | ENA
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