Sort   by:  
 Page size 
TranscriptomicsMapping and characterization of promoter-binding sites of protein phosphatase 1
Protein phosphatase 1 (PP1) is a Ser/Thr phosphatase that has been implicated in many key cellular functions including transcriptional regulation. Due to its involvement these many processes, it becomes difficult to directly link PP1 to transcriptional regulation on the chromatin level as no direct ...
ORGANISM(S): Homo sapiens 
2015-06-17 | E-GEOD-54170 | biostudies-arrayexpress
TranscriptomicsA library of yeast transcription factor motifs
The sequence specificity of DNA-binding proteins is the primary mechanism by which the cell recognizes genomic features. Here, we describe systematic determination of yeast transcription factor DNA-binding specificities. We obtained binding specificities for 112 DNA-binding proteins representing 19 ...
ORGANISM(S): Saccharomyces cerevisiae 
2008-12-26 | E-GEOD-12349 | biostudies-arrayexpress
ProteomicsAnalysis of proteins that bind promoter of the PDR5 multidrug transporter gene in Saccharomyces cerevisiae using minichromosome isolation and LC-MS/MS
The Pleiotropic Drug Resistance (PDR) network is central to the drug response in fungi, and its overactivation is associated with drug resistance. However, gene regulation of the PDR network is not well understood. Here, we established a method to identify proteins that bind promoter of the PDR5 mul...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-02-22 | PXD028798 | Pride
Lc-msms Minichromosome Promoter-binding proteins Immunoprecipitation Saccharomyces cerevisiae 
GenomicsHuman RNA polymerase III transcriptomes and relationships to Pol II promoter chromatin and enhancer-binding factors.
RNA polymerase (Pol) III transcribes many noncoding RNAs (for example, transfer RNAs) important for translational capacity and other functions. We localized Pol III, alternative TFIIIB complexes (BRF1 or BRF2) and TFIIIC in HeLa cells to determine the Pol III transcriptome, define gene classes and r...
ORGANISM(S): Homo sapiens 
2010-04-14 | E-GEOD-20309 | biostudies-arrayexpress
ProteomicsAnalysis of E2F2 interacting proteins
In an attempt to identify proteins interacting with transcription factor E2F2, proteins extracted from HA-E2F2 overexpressing HEK293T cells were were subjected to immunoprecipitation (IP) with an anti-HA antibody or, as a negative control (NegCt), with an anti-T antibody. Immunoprecipitated proteins...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-03-09 | MSV000079567 | MassIVE
TranscriptomicsSR Proteins Collaborate with 7SK and Promoter-Associated Nascent RNA to Release Paused Polymerase
RNAP II is frequently paused near gene promoters in mammals and its transition to productive elongation requires active recruitment of P-TEFb, a cyclin-dependent kinase for RNAP II and other key transcription elongation factors. A fraction of P-TEFb is sequestered in an inhibitory complex containing...
ORGANISM(S): Mus musculus 
2013-05-09 | E-GEOD-45517 | biostudies-arrayexpress
TranscriptomicsMapping of EZH2-WT and EZH2-mTP5 binding sites to human promoter regions
To gain genome wide information on the association of EZH2 with promoter regions in HeLa cells, DamID experiments and subsequent analysis by promoter arrays (Affymetrix GeneChip Human Promoter 1.0R ) were performed. The DamID method uses fusions of the bacterial Dam DNA methylase and the protein of ...
ORGANISM(S): Homo sapiens 
2012-11-13 | E-GEOD-39593 | biostudies-arrayexpress
GenomicsDNA specificity determinants associate with distinct transcription factor functions
To elucidate how genomic sequences build transcriptional control networks we need to understand the connection between DNA sequence and transcription factor binding and function. Binding predictions based solely on consensus predictions are limited because a single factor can use degenerate sequenc...
ORGANISM(S): Homo sapiens 
2009-12-18 | E-GEOD-17954 | biostudies-arrayexpress
TranscriptomicsGenome-wide binding of replication initiation proteins in Bacillus subtilis
Initiation of DNA replication requires binding of the initiator protein, DnaA, to specific binding sites in the chromosomal origin of replication, oriC. In low G+C Gram-positive bacteria, the primosomal proteins DnaD and DnaB, in conjunction with loader ATPase DnaI, load the replicative helicase at ...
ORGANISM(S): Bacillus subtilis 
2010-12-07 | E-GEOD-23686 | biostudies-arrayexpress
GenomicsModulation of enhancer looping and differential gene targeting by Epstein-Barr virus transcription factors directs epigenetic reprogramming
Epstein-Barr virus (EBV) epigenetically reprogrammes B-lymphocytes to drive immortalization and facilitate viral persistence. Host-cell transcription is perturbed principally through the actions of EBV EBNA 2, 3A, 3B and 3C, with cellular genes deregulated by specific combinations of these EBNAs thr...
ORGANISM(S): Homo sapiens 
2013-09-13 | E-GEOD-47629 | biostudies-arrayexpress
Sort   by:  
 Page size