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MetabolomicsIntegrating two chromatographies in a co-fractionation mass spectrometry approach improved protein-metabolite interactions (PMIs) mapping capabilities and revealed novel protein-ligand pairings in Escherichia coli.
The protein-metabolite interactome remains understudied. We previously developed a co-fractionation mass-spectrometry-based approach called PROMIS to address this gap. PROMIS relies on size separation chromatography of protein-metabolite complexes from lysates, followed by proteomics and metabolomic...
2025-04-25 | MTBLS9098 | MetaboLights
ProteomicsApplicability of chromatographic co-elution for antibiotic research
Identification of the molecular target is crucial for evaluating novel antibiotics. To support target identification, a label-free method based on chromatographic co-elution (TICC) has been developed previously. In TICC, an alteration in the elution profile of the target-bound drug compared to free ...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2020-10-01 | PXD017640 | Pride
Essential proteins Bacillus subtilis Protein complexes Protein-protein interaction 
UnknownA rapid and accurate approach for prediction of interactomes from co-elution data (PrInCE).
Not available
S-EPMC5654062 | biostudies-literature
ProteomicsSystem-wide profiling of protein-complexes via size exclusion chromatography– mass spectrometry (SEC-MS)
In living cells most proteins are organized in stable or transient functional assemblies known as protein complexes, which control a multitude of vital cellular processes such as cell cycle progression, metabolism, and signal transduction. System-wide workflows for analysis of protein complexes usin...
ORGANISM(S): Homo sapiens (Human) 
2022-05-06 | PXD018033 | Pride
Protein complex analysis Sec-ms Protein co-elution profiling 
MetabolomicsUPLC-MS-Based Serum Metabolic Profiling Reveals Potential Biomarkers for Predicting Propofol Responsiveness in Females

Although previous studies have shown that certain factors interfere with the sensitivity of propofol, the mechanisms for interindividual variability in response to propofol remain unclear. This study aimed to screen the metabolites to predict patients' sensitivity to propofol and to identify meta...

2021-09-29 | MTBLS2311 | MetaboLights
UnknownAnalysis of Human Nuclear Protein Complexes by Quantitative Mass Spectrometry Profiling.
Not available
S-EPMC6387628 | biostudies-literature
ProteomicsSystematic analysis of human nuclear protein complexes by quantitative mass spectrometry profiling
Many proteins form stable complexes that coordinate diverse and complex cellular processes. Systematic analysis of protein complexes provides insights into how the ensemble of expressed proteome is organized into functional units. Protein complexes are usually identified using either yeast-two-hybri...
ORGANISM(S): Human Gbm Cell Lines 
MSV000081520 | MassIVE
ProteomicsSEC-SWATH-MS HEK293 - Complex-centric proteome profiling by SEC-SWATH-MS
Increasingly, biochemical co-fractionation-based approaches are used to study interactomes and protein complexes at high throughput. The devised methods facilitate the qualitative assignment and prediction of hundreds of putative cellular assemblies in one experiment and without dependency on geneti...
ORGANISM(S): Homo sapiens (Human) 
2019-01-11 | PXD007038 | Pride
Size exclusion chromatography Mass spectrometry Swath Protein complexes 
UnknownDeep interactome profiling of membrane proteins by co-interacting protein identification technology.
Not available
S-EPMC5444904 | biostudies-literature
TranscriptomicsRibosome profiling with MN and RNaseI
The aim of this experiment is to compare nucleases used in ribosome profiling using Drosophila melanogaster Kc167 cells and human U2OS osteosarcoma cells. Ribosome profiling was performed from nuclease digested samples using sucrose cushion centrifugation separated ribosomes.
ORGANISM(S): Homo sapiens 
2015-01-08 | E-MTAB-2421 | biostudies-arrayexpress
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