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The experiment uses HEK-293 Flp-In T-Rex cells to express fusion protein that combines ATP synthase subunit ATP5MG with a tandem fluorescent protein timer (tFT). The tFT tag combines mCherry and super folder GFP (sfGFP) fluorescent proteins. An exceptionally stable structure of sfGFP interferes with...
ORGANISM(S): Homo sapiens (Human) 
2024-03-08 | PXD038583 | Pride
Mitochondrial biogenesis requires the import of >1,000 mitochondrial preproteins from the cytosol. Most studies on mitochondrial protein import are focused on the core import machinery. Whether and how the biophysical properties of substrate preproteins affect overall import efficiency is underexplo...
ORGANISM(S): Mus musculus 
2023-03-17 | GSE227295 | GEO
Mitochondrial protein import clogging as a mechanism of disease
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