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ProteomicsA protein microarray-based analysis of S-nitrosylation
Yeast protein microarrays were utilized to investigate determinants of S-nitrosylation by biologically relevant low-mass S-nitrosothiols (SNOs). Large numbers of S-nitrosylated yeast proteins were identified after treatment with SNOs, among which those with active-site Cys thiols residing at N termi...
ORGANISM(S): Saccharomyces cerevisiae 
2010-05-19 | E-GEOD-19218 | biostudies-arrayexpress
ProteomicsChemoselective umpolung of thiols to episulfoniums for cysteine bioconjugation
Modification of native cysteine is an important tool in protein research and relies on fast and chemoselective reactions under bioambient conditions. Nucleophilic cysteine can be functionalized with electrophiles, such as maleimides, that carry specific chemical groups, depending on the desired appl...
ORGANISM(S): Escherichia Coli 
2023-12-20 | PXD038723 | JPOST Repository
UnknownPrediction of reversibly oxidized protein cysteine thiols using protein structure properties.
Not available
S-EPMC2248312 | biostudies-literature
ProteomicsAn Anti-Cancer Gold(III)-Activated Porphyrin Scaffold that Covalently Modifies Protein Cysteine Thiols
Cysteine thiols of many cancer-associated proteins are attractive targets of anti-cancer agents. Herein, we unequivocally demonstrate a distinct thiol-targeting property of gold(III) mesoporphyrin IX dimethyl ester (AuMesoIX) and its anti-cancer activities. While the binding of cysteine thiols with ...
ORGANISM(S): Homo sapiens (Human) 
2021-09-08 | PXD016875 | Pride
Gold • porphyrin • ligand reactivity• cysteine thiol conjugation • anti-tumor agents 
ProteomicsQuantitative analysis of redox proteome reveals protein thiol switches regulating fundamental processes in developmental haematopoiesis
Foetal and adult hematopoietic stem and progenitor cells (HSPCs) are characterized by distinct redox homeostasis that may influence their differential cellular behaviour in normal and malignant haematopoiesis. In this work, we have applied a quantitative mass spectrometry-based redox proteomic appro...
ORGANISM(S): Mus musculus (Mouse) 
2022-06-09 | PXD030043 | Pride
Hematopoiesis Leukemia Oxidative modifications Redox proteomics 
UnknownAn anticancer gold(III)-activated porphyrin scaffold that covalently modifies protein cysteine thiols.
Not available
S-EPMC6983449 | biostudies-literature
ProteomicsQuantification of the percentage of oxidized cysteine thiols in GrxS12 under normal and H2O2 treatments.
To evaluate the percentage of oxidized cysteine thiols, the GrxS12 proteins underwent either with or without H2O2 treatment were alkylated by iodoacetamide (IAM), reduced with DTT, and subsequently the newly generated free Cys were alkylated with N-ethylmaleimide (NEM). The spectrometric analysis of...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-01-16 | PXD059065 | Pride
Grxs12 H2o2 Oxidized cysteine thiols Quantification 
UnknownProtein vicinal thiols as intrinsic probes of brain redox states in health, aging, and ischemia.
Not available
S-EPMC11233328 | biostudies-literature
UnknownCysteine trisulfide oxidizes protein thiols and induces electrophilic stress in human cells.
Not available
S-EPMC8497997 | biostudies-literature
UnknownModulation of cell surface protein free thiols: a potential novel mechanism of action of the sesquiterpene lactone parthenolide.
Not available
S-EPMC2780735 | biostudies-literature
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