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MetabolomicsProtein-metabolite interactomics of carbohydrate metabolism reveal regulation of lactate dehydrogenase

Metabolic networks are interconnected and influence diverse cellular processes. The protein-metabolite interactions that mediate these networks are frequently low affinity and challenging to systematically discover. We developed mass spectrometry integrated with equilibrium dialysis for the disco...

2023-08-07 | MTBLS7237 | MetaboLights
MetabolomicsPROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana
Small molecules not only represent cellular building blocks and metabolic intermediates, but also regulatory ligands and signaling molecules that interact with proteins. Although these interactions affect cellular metabolism, growth, and development, they have been largely understudied. Herein, we d...
2018-07-19 | MTBLS94 | MetaboLights
MetabolomicsIntegrating two chromatographies in a co-fractionation mass spectrometry approach improved protein-metabolite interactions (PMIs) mapping capabilities and revealed novel protein-ligand pairings in Escherichia coli.
The protein-metabolite interactome remains understudied. We previously developed a co-fractionation mass-spectrometry-based approach called PROMIS to address this gap. PROMIS relies on size separation chromatography of protein-metabolite complexes from lysates, followed by proteomics and metabolomic...
2025-04-25 | MTBLS9098 | MetaboLights
MetabolomicsTranscript, protein and metabolite temporal dynamics in the CAM plant Agave
Already a proven mechanism for drought sustainability, crassulacean acid metabolism (CAM) is a specialized type of photosynthesis that maximizes water-use efficiency (WUE) via an inverse (compared to C3 and C4 photosynthesis-performing species) day/night pattern of stomatal closure/opening to shift ...
2017-10-13 | MTBLS363 | MetaboLights
MetabolomicsIntegration of magnetic resonance imaging and protein and metabolite CSF measurements to enable early diagnosis of secondary progressive multiple sclerosis

Molecular networks in neurological diseases are complex. Despite this fact, contemporary biomarkers are in most cases interpreted in isolation, leading to a significant loss of information and power. We present an analytical approach to scrutinize and combine information from biomarkers originati...

2018-08-13 | MTBLS558 | MetaboLights
MetabolomicsNew plasma preparation approach to enrich metabolome coverage in untargeted metabolomics: plasma protein bound hydrophobic metabolite release with proteinase K
Plasma untargeted metabolomics is a common method for evaluation of the mechanisms underlying human pathologies and identification of novel biomarkers. The plasma proteins provide the environment for transport of hydrophobic metabolites. The current sample preparation protocol relies on the immediat...
2022-03-18 | MTBLS670 | MetaboLights
MetabolomicsWalnut protein hydrolysate alleviates neuroinflammation and memory impairment in naturally aged mice via a gut microbiota-metabolite-neuroinflammation axis
This study investigated age-associated alterations in the serum metabolome and the metabolic effects of walnut protein hydrolysate (WPH) supplementation in naturally aged mice. Male C57BL/6J mice at different ages (2-month, 10-month and 18-month) were used to characterize metabolic changes associate...
2026-09-04 | MTBLS15363 | MetaboLights
ProteomicsDynamic maps of protein-protein-metabolite complexes in S. cerevisiae
The project aims to create dynamic maps of protein-protein-metabolite complexes in S. cerevisiae across growth phases using PROMIS (PROtein–Metabolite Interactions using Size separation). It is a biochemical, untargeted, proteome- and metabolome-wide method to study protein-protein and protein–metab...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-10-25 | PXD026639 | Pride
Protein-metabolite complexes Ligand Protein-protein complexes 
ProteomicsDiscovery of Regulatory Complexes in Arabidopsis via Protein–Metabolite Mapping During Pseudomonas Infection
Protein–metabolite interactions (PMIs) are critical regulators of cellular processes, yet their roles in plant–pathogen interactions remain poorly understood. Here, we applied PROMIS (Protein–Metabolite Interactions using Size separation) to map the Arabidopsis interactome during infection with Pseu...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Pseudomonas syringae pv. tomato str. DC3000 Bacteria 
2026-05-05 | PXD069625 | Pride
Nata1 Promis Protein-metabolite interactions 
ProteomicsDynamic maps of protein-protein-metabolite complexes of Arabidopsis Thaliana seedlings
The project aimed to create dynamic maps of protein-protein-metabolite complexes of Arabidopsis thaliana seedlings using PROMIS (PROtein–Metabolite Interactions using Size separation). The approach involves using size exclusion chromatography (SEC) to separate complexes, followed by LC-MS-based pro...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-10-14 | PXD026713 | Pride
Protein-metabolite complexes Ligand Protein-protein complexes 
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