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TranscriptomicsTranscription profiling of two human colon cancer cell lines derived from a primry tumor and a lypmh node metastatsis from a single patient reveals global alterations in mRNA polysomal recruitment in a cell model of colorectal cancer progression to metastasis.
Two colon cancer cell lines are under study. SW480 and SW620. The first one is derived from primary cancer, SW620 are from lymphnode metastatic sites. they both come from the sample patient. Polysomal RNA fractions from the two isogenic colon cancer cells lines was purified by sucrose gradient and h...
ORGANISM(S): Homo sapiens 
2007-10-27 | E-GEOD-2509 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human colorectal cancer cell lines derived from a primary tumor and a corresponding lymph node metastasis from the same individual
SW480 and SW620 are colon cancer cells lines derived from a primary tumor and a corresponding metastasis from the same individual. The numbers indicate the three independent replicate RNA samples processed. Three different software packages were used in parallel for signal calculation: Affymetrix mi...
ORGANISM(S): Homo sapiens 
2007-08-04 | E-GEOD-1323 | biostudies-arrayexpress
TranscriptomicsJNK1 Ablation in Mice Confers Long-term Metabolic Protection from Diet-Induced Obesity at the Cost of Increased Skin Oxidative Damage
Obesity and insulin resistance are associated with oxidative stress, which may be implicated in their progression. The kinase JNK1 emerged as a promising drug target for the treatment of obesity and type-2 diabetes. However, JNK1 is a key mediator of the oxidative stress response, promoting either c...
ORGANISM(S): Mus musculus 
2016-09-01 | E-GEOD-73759 | biostudies-arrayexpress
ProteomicsNAMPT and NNMT released via extracellular vesicles and as soluble mediators are distinguished traits of BRAF inhibitor resistance of melanoma cells impacting on the tumor microenvironment
Drugs targeting mutant oncogenes are effective, even though resistance rapidly develops. This complex picture includes acquired intrinsic tumor and tumor microenvironmental -mediated mechanisms. Here we showed that melanoma cells resistant to BRAF inhibitors (BRAFi) overexpressed the rate limiting e...
ORGANISM(S): Homo sapiens (Human) 
2025-07-28 | PXD063060 | Pride
Nampt  nnmt  metastatic melanoma  resistance  extracellular vesicles  secretome  signaling  tumor microenvironment 
ProteomicsYAP modulates mitochondrial function and sensitive to NAMPT inhibition in triple negative breast cancer cells
YAP and TAZ coordinate several NAD+-dependent processes like glycolysis, fatty acid oxidation and glutaminolysis in nutrient-deprivation conditions. However, correlations between NAD(H) levels and YAP/TAZ metabolic function are still poorly understood. We showed that stable silencing of YAP in the t...
ORGANISM(S): Homo sapiens (Human) 
2025-02-24 | PXD046817 | Pride
Mitochondrial fitness Metabolic adaptation Yap Drug resistance Fk866 Cell cycle Nad(h) 
TranscriptomicsTranslatome and transcriptome profiling of estradiol response in MCF7 cells
We subjected MCF7 cells to starvation with 0.5% charcoal treated serum for 48h and then we added 17-beta estradiol (E2) at final concentration of 10 nM, profiling before and after 60 minutes of treatment the transcriptome and the translatome, coming from the polysomal pool of mRNAs after sucrose gra...
ORGANISM(S): Homo sapiens 
2010-12-31 | E-GEOD-22012 | biostudies-arrayexpress
TranscriptomicsRIP-chip of HuR in MCF7 cells exposed to doxorubicin
The purpose of the study was to identify mRNA bound to HuR in the presence of doxorubicin in MCF7 cells. We collected cytoplasmic RNA from untreated and treated cells and detected differentially expressed genes (DEGs). We also coimmunoprecipitated HuR and IgG (as control) from doxorubicin treated ce...
ORGANISM(S): Homo sapiens 
2011-12-31 | E-GEOD-33055 | biostudies-arrayexpress
TranscriptomicsTranslatome and transcriptome profiling of FK866 response in Jurkat cells
We treated Jurkat cells for 48 hr with a sublethal dose of FK866 (5 nM) and DMSO (Mock, control treatment). RNA samples for microarrays derived from fractionated samples by sucrose gradient (sub-polysomes, polysomes), giving us the chance to perform an analysis among polysomal/subpolysomal distribu...
ORGANISM(S): Homo sapiens 
2015-04-01 | E-GEOD-53772 | biostudies-arrayexpress
TranscriptomicsRole of Per2 in the control of liver glycogen metabolism
To investigate the role of Per2 in glucose metabolism in-vivo we used mice bearing a targeted gene mutation in the Per2 gene (Per2brdm) and thus unable to express a functional Per2 protein. Mice were hosted in our standard mouse facility in a 12-hour light and 12-hour dark cycle. Wt and Per2brdm mic...
ORGANISM(S): Mus musculus 
2013-09-02 | E-GEOD-30139 | biostudies-arrayexpress
TranscriptomicsFasting potentiates the anticancer activity of Crizotinib
Background: Tyrosine kinase inhibitors (TKIs) are widely used for treating solid and hematologic malignancies. However, their efficacy is frequently short lived, warranting the search for safe potentiation strategies. Short courses of fasting were shown to sensitize cancer cells to chemo- and radiot...
ORGANISM(S): Homo sapiens 
2016-07-03 | E-GEOD-62663 | biostudies-arrayexpress
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