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ProteomicsIdentification of LC3-binding proteins on lipid droplets
In the search for LD-localized proteins that may mediate lipophagy, we purified LDs from HeLa cells treated with oleic acid (OA), which stimulates LD biogenesis and lipophagy, together with chloroquine (CQ), which inhibits lysosomal degradation. We incubated the LD lysates with purified GST-LC3B, an...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD036200 | Pride
Lipid droplet Lc3 
ProteomicsIdentification of phosphorylation sites of HA-ORP8 immunoprecipitated from cells
To candidate phosphorylation sites of ORP8 in cells and the stoichiometric change of ORP8's phosphorylation when AMPK is activated,LC-MS/MS analysis of ORP8 immunoprecipitated from serum-starved cells and cells treated with AMPK activator AICAR was performed.
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD036204 | Pride
Phosphorylation Orp8 
ProteomicsIdentification of phosphorylation sites of purified ORP8 from in vitro AMPK kinase assay
We investigated the potential role of AMPK in the phosphorylation of ORP8.In vitro kinase assay using purified ORP8 and kinase-active AMPK complex confirmed that ORP8 is the direct substrate of AMPK. Phosphorylated ORP8 from in vitro kinase assay was analyzed by HPLC-MS/MS.
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD036201 | Pride
Ampk Phosphorylation Orp8 
ProteomicsLactylation stabilizes TFEB to elevate autophagy and lysosomal activity
To identify the interaction proteins of TFEB, HEK293T cells transiently expressing TFEB-Flag were immunoprecipitated with anti-Flag beads. The beeds were used to do a LC/MS.
ORGANISM(S): Homo Sapiens 
2024-03-13 | PXD050578 |
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