Sort   by:  
 Page size 
UnknownRole of PUM RNA-Binding Proteins in Cancer.
Not available
S-EPMC7796173 | biostudies-literature
TranscriptomicsmRNAs associated with human PUM1 protein
To identify mRNAs associated with human PUM1 protein, we used a modified Ribonucleoprotein-ImmunoPrecipitation Microarray (RIP-Chip) approach on HeLa S3 cancer cells that express PUM1. PUM ribonucleoprotein (RNP) complexes were captured from cell-free extracts with anti PUM1 specific antibody Bethyl...
ORGANISM(S): Homo sapiens 
2008-09-08 | E-GEOD-12351 | biostudies-arrayexpress
TranscriptomicsmRNAs associated with human PUM2 protein
To identify mRNAs associated with human PUM2 protein, we used a modified Ribonucleoprotein-ImmunoPrecipitation Microarray (RIP-Chip) approach on HeLa S3 cancer cells that express PUM2. PUM ribonucleoprotein (RNP) complexes were captured from cell-free extracts with specific anti PUM2 antibody couple...
ORGANISM(S): Homo sapiens 
2008-09-08 | E-GEOD-12352 | biostudies-arrayexpress
ProteomicsThe interactome of flavin adenine dinucleotide (FAD) with RNA-binding proteins
We explored the interactome of flavin adenine dinucleotide (FAD) and the matrix of UV-immobilised FAD bound more than 3,000 proteins from SW-620 lysate. GO enrichment analysis showed that about 600 RNA-binding proteins were bound to FAD beads and, surprisingly, more than 40 proteins showed clear dos...
ORGANISM(S): Homo sapiens (Human) 
2022-10-06 | PXD036953 | Pride
Pum-rna Photo-immobilisation Fad Competition pulldown 
TranscriptomicsRIP-Chip of BRAT and PUM from 0-3 hour Drosophila melanogaster embryos
BRAT-associated mRNAs and PUM-associated mRNAs were identified in early Drosophila embryos by RNA co-immunoprecipitation of the endogenous proteins using synthetic antibodies, followed by microarray analysis (RIP-Chip). Nine RNA co-immunoprecipitations were performed. This includes 3 biological repl...
ORGANISM(S): Drosophila melanogaster 
2015-03-28 | E-GEOD-60466 | biostudies-arrayexpress
TranscriptomicsmRNAs associated with human PUM1 and PUM2 protein
This SuperSeries is composed of the following subset Series: GSE12351: mRNAs associated with human PUM1 protein GSE12352: mRNAs associated with human PUM2 protein Refer to individual Series
ORGANISM(S): Homo sapiens 
2008-09-08 | E-GEOD-12357 | biostudies-arrayexpress
TranscriptomicsNORAD-induced phase separation of PUMILIO proteins is required for genome maintenance
Long noncoding RNAs (lncRNAs) can regulate the activity of interacting RNA binding proteins (RBPs), but how lncRNAs efficiently compete against other transcripts to bind and regulate RBP activity is poorly understood. This problem is exemplified by NORAD, a lncRNA that maintains genomic stability by...
ORGANISM(S): Homo sapiens 
2021-03-25 | GSE154812 | GEO
TranscriptomicsPumilio regulates mRNA decay during the late maternal-to-zygotic transition in Drosophila melanogaster
Pumilio (PUM) is a Drosophila member of a conserved family of sequence-specific RNA-binding proteins that have been shown to regulate mRNA stability and/or translation in a variety of organisms. PUM has been shown to repress the translation of several mRNAs in the Drosophila early embryo; failure to...
ORGANISM(S): Drosophila melanogaster 
2017-07-01 | GSE48645 | GEO
TranscriptomicsRNA affinity isolation with TAP-Pum and controls
Biochemical purification: Five grams of adult flies or 2.5 g of embryos were used in each affinity purification. Flies or embryos were suspended in 15 ml of buffer B (buffer A plus 1.5 mM dithiothreitol [DTT], 1 mM phenylmethylsulfonylfluoride [PMSF], 0.5 5g/ml leupeptin, 0.8 5g/ml pepstatin, 20 U/m...
ORGANISM(S): Drosophila melanogaster 
2006-02-28 | E-GEOD-3582 | biostudies-arrayexpress
UnknownNORAD-induced Pumilio phase separation is required for genome stability.
Not available
S-EPMC8266761 | biostudies-literature
Sort   by:  
 Page size