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GenomicsWater purification system
Water purification system Raw sequence reads
PRJNA1086135 | ENA
TranscriptomicsA native chromatin purification system for epigenomic profiling in C. elegans
We describe an in vivo chromatin purification system for genome-wide epigenetic profiling in C. elegans. In this system, we coexpressed the E. coli biotin ligase enzyme (BirA), together with the C. elegans H3.3 gene fused to BioTag, a 23-amino acid peptide serving as a biotinylation substrate for Bi...
ORGANISM(S): Caenorhabditis elegans 
2010-05-19 | E-GEOD-18898 | biostudies-arrayexpress
TranscriptomicsThe Extracellular RNA Quality Control (exRNAQC) study: Small RNA sequencing of RNA purification kit study (exRNAQC011)
To evaluate the impact of the RNA purification method on extracellular RNA (exRNA) sequencing, 8 different RNA purification kits were compared by applying Small RNA sequencing (Illumina) to exRNA from human healthy donor plasma. Minimum and maximum plasma input volumes recommended by the manufacture...
ORGANISM(S): Homo sapiens 
2021-06-10 | E-MTAB-10505 | biostudies-arrayexpress
TranscriptomicsTissue-specific nuclei purification from animal models for genome-wide expression and chromatin profiling.
Here we describe a method for purifying nuclei from specific tissues of animal models that allows simultaneous analysis of both expression and chromatin profiles. The method is based on in vivo labeling of the nuclear envelope and subsequent affinity purification. We describe the use of the method t...
ORGANISM(S): Caenorhabditis elegans 
2011-12-05 | E-GEOD-31602 | biostudies-arrayexpress
UnknownAccelerating AAV purification process development using high-throughput resin tip module.
Not available
S-EPMC12696448 | biostudies-literature
GenomicsPoecilia mexicana strain:VI/17 Rio Purification
Poecilia mexicana strain:VI/17 Rio Purification Genome sequencing
PRJNA1398337 | ENA
ProteomicsLight-controlled affinity purification of protein complexes
Methods to affinity purify proteins are widely used in protein research. One important application is to identify interacting proteins of an affinity-purified protein of interest (POI) by mass spectrometry. Here, we developed an optogenetics-derived and light-controlled affinity purification method ...
ORGANISM(S): Homo sapiens (Human) 
2019-03-18 | PXD012156 | Pride
Affinity purification Mass spectrometry Optogenetics Protein-protein interaction Phytochrome Zap70 
ProteomicsxIP-MS: topological analysis of chromatin-associated protein complexes using single affinity purification
In recent years, cross-linking mass spectrometry (XL-MS) has proven to be a robust and effective method of interrogating macromolecular protein complex topologies at peptide resolution. Traditionally, XL-MS workflows have utilized homogenous complexes obtained through time-limiting reconstitution, t...
ORGANISM(S): Homo sapiens (Human) 
2015-11-17 | PXD002987 | Pride
Affinity purification Chemical cross-linking mass-spectrometry Chromatin 
UnknownAn Integrated Approach to Aggregate Control for Therapeutic Bispecific Antibodies Using an Improved Three Column Mab Platform-Like Purification Process.
Not available
S-EPMC6667909 | biostudies-literature
GenomicsPurification of pancreatic endocrine subsets from mouse islets
Purification of pancreatic endocrine subsets from mouse islets
PRJNA628871 | ENA
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