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RNA isolation and RNA-Seq: Wild-type Xcc or pXccBphP strains were cultured in far-red light or dark conditions up to logarithmic phase (0.7 to 0.8 OD600) at 28C in PYM broth. Total bacterial RNA was isolated using the MasterPureTM RNA Purification Kit (Epicentre, Illumina). Samples corresponding to ...
ORGANISM(S): Xanthomonas campestris pv. campestris 
We assess the prevalence of polygenic evolution in pathways between the yeasts S. cerevisiae and S. bayanus. We first established short-read sequencing methods to detect cis-regulatory variation in a diploid hybrid between the species. We then formulated an analytic strategy to test for the scenar...
ORGANISM(S): Saccharomyces bayanus x Saccharomyces cerevisiae 
Sister chromatid cohesion, established during replication by the protein complex Cohesin, is essential for both chromosome segregation and double-strand break (DSB) repair. Normally cohesion formation is strictly limited to the S-phase of the cell cycle, but DSBs can trigger cohesion also after DNA ...
ORGANISM(S): Saccharomyces cerevisiae 
While developmental processes such as axon pathfinding and synapse formation have been characterized in detail, comparatively less is known of the intrinsic developmental mechanisms that regulate transcription of ion channel genes in embryonic neurons. Early decisions, including motoneuron axon targ...
ORGANISM(S): Drosophila melanogaster 
Sky1 is a Saccharomyces cerevisiae rich serine-arginine (SR) protein-specific kinase and its enzymatic activity is essential in the cytotoxicity caused by cisplatin, although the molecular mechanisms supporting this function are not understood. We present a transcriptome analysis discriminating betw...
ORGANISM(S): Saccharomyces cerevisiae 
In metazoans, mRNA quality is tightly monitored from transcription to translation. A key role lies with the exon junction complex (EJC) that is placed upstream of the exon-exon junction after splicing. The EJC inner core is composed of Magoh, Y14, eIF4AIII and BTZ and the outer core of proteins invo...
ORGANISM(S): Trypanosoma brucei 
2024-03-25 | PXD050946 | Pride
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