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To ensure that the pool of precursor amino acids utilized for protein synthesis is completely labeled prior to the first labeling time-point, we conducted an isotopomer analysis. After adaption to media, cells were plated at a density of 500,000 cells per 10 cm plate. 8 days after plating, the confl...
ORGANISM(S): Homo sapiens (Human) 
2016-06-14 | PXD003559 | Pride
We used a chronic unpredicted mild stress (CUMS) protocol to separate depression-susceptible and stress-insensitive rat subgroups. Differential proteomes in the hippocampus were analyzed based on isobaric tags for relative and absolute quantitation (iTRAQ) labeling combined with mass spectrometry.
ORGANISM(S): Rattus norvegicus (Rat) 
2022-02-17 | PXD025773 | Pride
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