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ProteomicsA novel pulse-chase SILAC strategy measures changes in protein decay and synthesis rates induced by perturbation of proteostasis with an Hsp90 inhibitor.
We developed a pulse-chase variant of SILAC (stable isotope labeling by amino acids in cell culture pcSILAC). pcSILAC can quantitate in one experiment and for two conditions the relative levels of proteins newly synthesized in a given time as well as the relative levels of remaining preexisting prot...
ORGANISM(S): Homo sapiens (Human) 
2013-12-10 | PXD000538 | Pride
Hsp90 T-cells Pulse-chase Silac Synthesis rate Decay rate 
ProteomicsPhosphorylation of Hsp90 on serine residues in the charged linker modulates binding to interacting proteins and has implications for overall Hsp90β conformation (limited proteolysis experiment)
The charged linker region in Hsp90 has been shown to be important for Hsp90 function and global conformation. In humans, two serines located in the charged linker region are constitutively phosphorylated in vivo. In order to gain knowledge on the possible effect of these phosphorylation sites on Hsp...
ORGANISM(S): Homo sapiens (Human) 
2021-07-07 | PXD025878 | Pride
Hsp90 Limited proteolysis Charged linker Phosphorylation 
ProteomicsPhosphorylation of Hsp90β on serine residues in the charged linker modulates binding to interacting proteins and has implications for overall Hsp90β conformation (Occupancy and phospho-modulation data)
Human Hsp90β is constitutively phosphorylated in vivo on two serine residues in the charged linker region, S226 and S255. We developed a targeted method to measure the phosphorylation occupancy (the extent of phosphorylation) of both phosphosites in a range of cultured cell lines and consistently fo...
ORGANISM(S): Homo sapiens (Human) 
2021-07-07 | PXD025888 | Pride
Hsp90 Heat shock Tio Stress Hsp90 inhibitors Phosphorylation Occupancy 
ProteomicsA reference database of electrophoretic migration patterns for human proteins in a set of cell lines.
Protein denaturing electrophoresis based on sodium dodecyl sulfate (SDS-PAGE) is a technique widely applied for studying proteins, either alone or in combination with specific antibody-based detection. Despite its popularity, SDS-PAGE and western blotting are poorly reproducible and can give differe...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2023-01-05 | PXD026750 | Pride
Gelc-ms Sds-page Molecular weight Electrophoresis Western blot 
ProteomicsPhosphorylation of Hsp90 on serine residues in the charged linker modulates binding to interacting proteins and has implications for overall Hsp90β conformation
Serine residues in the charged linker region of human Hsp90beta are highly phoshporylated in vivo. Mutation of these residues to alanines resulted in altered binding of many Hsp90beta interactors. This project contains the data and searches related to co-immunoprecipitation experiments with wild-typ...
ORGANISM(S): Homo sapiens (Human) 
2021-07-07 | PXD025873 | Pride
Hsp90 Phosphorylation Interactome 
ProteomicsDynamics of Proteo-Transcriptomic Response to HIV-1 Infection
We have investigated the dynamic host response to HIV-1 infection by systematically measuring transcriptome, proteome and phosphoproteome expression changes in infected and uninfected SupT1 CD4+ T cells at 5 time-points throughout the HIV-1 replication cycle (from 2h to 24h).
ORGANISM(S): Homo sapiens (Human) Human immunodeficiency virus type 1 3916-CRO 
2019-01-22 | PXD005810 | Pride
Human Phosphoprotein Proteomics Viral replication T-cells Hiv Infection Transcriptomics 
ProteomicsHsp90 inhibition induces both protein-specific and global changes in the ubiquitinome
We used stable isotope labeling and antibody-based peptide enrichment to quantify more than 1500 protein sites modified with a Gly-Gly motif, the remnant of ubiquitination, in human T-cells treated with an Hsp90 inhibitor. We compared the data with the evolution of net protein levels in the same cel...
ORGANISM(S): Homo sapiens (Human) 
2015-03-19 | PXD001549 | Pride
Mass spectrometry Hsp90 Stress Silac Protein synthesis Ubiquitin Quantitation Chaperones Protein folding Protein degradation 
ProteomicsDynamic impacts of the inhibition of the molecular chaperone Hsp90 on the T-cell proteome have implications for anti-cancer therapy.
Using an integrated systematic approach to analyse the effects of Hsp90 inhibition in T-cells, we quantified differential changes in the Hsp90-dependent proteome, Hsp90 interactome, and a selection of the transcriptome. Kinetic behaviours in the Hsp90-dependent proteome were assessed using a novel p...
ORGANISM(S): Homo sapiens (Human) 
2013-12-10 | PXD000537 | Pride
Hsp90 T-cells Silac 
ProteomicsPhosphorylation of OSBL8 protein in interphase vs mitotic human cells
Phosphorylation of OSBL8 human protein was compared in interphase vs mitotic Hela cells with a new software developed in-house, Msviz, a graphical software tool developed for in-depth manual validation and quantitation of post-translational modifications.
ORGANISM(S): Homo sapiens (Human) 
2017-07-03 | PXD006254 | Pride
Osbl8 Tandem mass spectrometry Human Phosphorylation Qexactive 
ProteomicsQuantitative proteomics of rat livers shows that unrestricted feeding and re-feeding after food restriction are stressful for cellular proteostasis
We used quantitative MS-based proteomic analysis of whole rat livers to study the molecular events associated with a food restriction regimen as compared with ad libitum food and animals re-fed after a restriction time.
ORGANISM(S): Rattus norvegicus (Rat) 
2016-12-23 | PXD004068 | Pride
Food restriction Liver Rat Food intake Label-free 
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