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Genomewide measurements of whole embryo mRNA decay in Drosophila

whole embryo (all tissues) measurement of mRNA decay by 4-thiouridine pulse-chase These TU-Decay microarrays analyze mRNA levels at three timepoints: a one hour pulse, one hour chase, and three hour chase. Measurements with or without transcription inhibition by actinomycin D (ActD) were compared.

ORGANISM(S): Drosophila melanogaster

2015-03-31 | E-GEOD-67435 | biostudies-arrayexpress

Thiouracil Pulse-chase, Tachyzoites and Bradyzoites mRNA stability data

Pulse chase measurements using thiouracil (DTU) labeling via UPRT and chasing with uracil Data from tachyzoites is labeled "DTU Pulse Chase". Two independent pulse chase experiments were performed in tachyzoites, pulse chase 1 and 2. Duplicate arrays at each timepoint were performed for pulse chase ...

ORGANISM(S): Toxoplasma gondii

2005-07-16 | GSE2947 | GEO

Genomewide measurements of neural-specific mRNA decay in Drosophila embryos

Neural-specific mRNA decay measurements by TU-Decay technique in control and Pumilio knockdown embryos These TU-Decay microarrays analyze mRNA levels at three timepoints: a one hour pulse, one hour chase, and three hour chase. Neural-specific RNA purification was achieved using prospero-GAL4 drivin...

ORGANISM(S): Drosophila melanogaster

2015-04-02 | E-GEOD-67512 | biostudies-arrayexpress

Thiouracil Pulse-chase, Tachyzoites and Bradyzoites mRNA stability data

ORGANISM(S): Toxoplasma gondii

2005-07-16 | E-GEOD-2947 | biostudies-arrayexpress

EC_tagging pulse-chase to measure mRNA decay in Drosophila larval neuroblasts and neurons

PRJNA883697 | ENA

Transcription profiling of Toxoplasma gondii thiouracil pulse-chase, tachyzoites and bradyzoites mRNA stability data

ORGANISM(S): Toxoplasma gondii

2005-07-07 | E-SMDB-2949 | biostudies-arrayexpress

A novel pulse-chase SILAC strategy measures changes in protein decay and synthesis rates induced by perturbation of proteostasis with an Hsp90 inhibitor.

We developed a pulse-chase variant of SILAC (stable isotope labeling by amino acids in cell culture pcSILAC). pcSILAC can quantitate in one experiment and for two conditions the relative levels of proteins newly synthesized in a given time as well as the relative levels of remaining preexisting prot...

ORGANISM(S): Homo sapiens (Human)

2013-12-10 | PXD000538 | Pride

In vivo pulse-chase in C. elegans reveals intestinal histone turnover changes upon starvation

PRJNA1224425 | ENA

3T3-L1 adipocyte protein turnover

Insulin is a potent regulator of protein metabolism. Here we describe a time-resolved map of insulin-regulated protein turnover in 3T3-L1 adipocytes using metabolic pulse-chase labelling and high-resolution mass spectrometry.

ORGANISM(S): Mus musculus (Mouse)

2016-10-07 | PXD003696 | Pride

Proteostasis Pulse-chase silac Protein synthesis Insulin

EC_tagging pulse-chase to measure mRNA decay in Drosophila larval neuroblasts and neurons

Nascent RNA was tagged with EU, via EC feeding, in targeted cells (neuroblasts or neurons) then purified after the initial pulse labeling or after a chase using media containing excess unmodified uridine

ORGANISM(S): Drosophila melanogaster

2022-09-26 | GSE214062 | GEO

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