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ProteomicsNewly synthesized proteome analysis of EPP2 cells, with combinations of induced Ctu1- or Elp3 KO and DMSO-, Torin1- and Rapamycin treatments
Newly synthesized proteome analysis of EPP2 murine pancreatic cancer cells with induced knockout of tRNA wobble enzymes Elp3 or Ctu1 and mTOR inhibitor treatment. Knockouts were induced for 4 days, mTOR inhibitors were 5 h rapamycin [50 nM] or torin 1 [50 nM].
ORGANISM(S): Mus musculus (Mouse) 
2025-05-07 | PXD047316 | Pride
Silac Plexdia Quanpa 
UnknownAn integrated workflow for quantitative analysis of the newly synthesized proteome.
Not available
S-EPMC10716174 | biostudies-literature
ProteomicsNewly synthesized proteome analysis of interferon gamma treated Hela cells
Using a newly-developed workflow for quantitative newly synthesized proteome analysis (QuaNPA), featuring automated sample processing and multiplexed DIA (plexDIA) analysis, changes in the newly synthesized proteome of IFN-gamma treated Hela cells were monitored over time.
ORGANISM(S): Homo sapiens (Human) 
2023-10-19 | PXD038915 | Pride
Dia Nascent proteome analysis Automated sample processing Interferon gamma Silac 
ProteomicsApplying plexDIA analysis to newly synthesized proteome samples generated using a semi-automated enrichment protocol
Samples generated with a newly development semi-automated enrichment protocol for newly synthesized proteins, using different amounts of protein input, were analysed using data-independent acquisition (DIA) and processed with the plexDIA software features of DIA-NN (https://www.nature.com/articles/s...
ORGANISM(S): Homo sapiens (Human) 
2023-10-19 | PXD043817 | Pride
Silac Plexdia Quanpa 
ProteomicsNascent proteome analysis of LN229 cells with Trp-starvation
Nascent proteome analysis of LN229 cells grown in tryptophan (Trp) depleted or standard cell culture medium for 10h
ORGANISM(S): Homo sapiens (Human) 
2025-12-04 | PXD067012 | Pride
Trp-starvation Silac Newly-synthesized proteins Quanpa 
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