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ProteomicsAnalysis of LDL-associated proteins using the method of digitized native protein mapping
The method of digitized native protein mapping, combining nondenaturing micro 2DE, grid gel-cutting and quantitative LC-MS/MS (in data-independent acquisition mode, or MSE), was improved by using a new MS/MS mode, ion mobility separation enhanced-MSE (HDMSE) and applied to analyze the area of human ...
ORGANISM(S): Homo sapiens (Human) 
2016-05-17 | PXD003720 | Pride
Grid gel-cutting Quantitative lc-hdmse Human plasma low-density lipoprotein Protein-protein interactions Nondenaturing micro 2de Digitized native protein mapping 
ProteomicsQuantitative proteomic analysis of the Toxoplasma gondii cytoskeleton and bioinformatic identification of highly antigenic proteins
The Toxoplasma gondii cytoskeleton is a highly organized structure essential for parasite motility, replication, and host cell invasion. To identify its components, a highly enriched fraction of tachyzoite cytoskeletons was obtained and quantitatively analyzed by mass spectrometry (LC/ESI/HDMSE). We...
ORGANISM(S): Eukaryota Toxoplasma gondii RH 
2025-08-04 | PXD063409 | Pride
Toxoplasma gondii Quantitative mass spectrometry Immunogenic peptides Cytoskeleton 
ProteomicsQuantitative Proteomic Analysis in Alveolar Type II Cells Reveals the Different Capacities of RAS and TGF-β to Induce Epithelial–Mesenchymal Transition
Alveolar type II (ATII) epithelial cells function as stem cells, contributing to alveolar renewal, repair and cancer. Therefore, they are a highly relevant model for studying lung diseases, including acute injury, fibrosis and cancer, in which signals transduced by RAS and transforming growth factor...
ORGANISM(S): Homo sapiens (Human) 
2021-02-04 | PXD023720 | Pride
Human top-down alveolar type ii epithelial cells 
UnknownQuantitative Proteomic Analysis in Alveolar Type II Cells Reveals the Different Capacities of RAS and TGF-β to Induce Epithelial-Mesenchymal Transition.
Not available
S-EPMC8048883 | biostudies-literature
ProteomicsProteomic analysis of cellular soluble proteins from human bronchial smooth muscle cells by combining nondenaturing micro 2DE and quantitative LC-MS/MS: Preparation of more than 4000 native protein maps
Soluble proteins of human bronchial smooth muscle cells (HBSMC) were separated by nondenaturing micro 2DE and a 30 mm X 40 mm area of the CBB-stained slab gel (1.0 mm thick) was cut into 1.1 mm X 1.1 mm squares, then the proteins in the 972 gel pieces (squares) were applied to quantitative LC-MS/MS....
ORGANISM(S): Homo sapiens (Human) 
2015-08-10 | PXD001471 | Pride
Cellular proteins Native protein map Nondenaturing micro 2de Lc-ms/ms Quantitative protein-protein interaction 
ProteomicsBloodstream infection N-glycoproteomics
Here we have performed targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports. Three hundred and sixty eight potential N-glycopeptides were quantified by mass...
ORGANISM(S): Homo sapiens (Human) 
2018-04-09 | PXD009048 | Pride
Sepsis Human plasma Bloodstream infection N-glycoproteomics 
ProteomicsLabel-free quantitative proteomic analysis of DNA methyltransferase mutants of the cyanobacterium Synechocystis sp. PCC 6803 – Analysis of total protein extracts
Total protein and membrane-enriched fractions of Synechocystis sp. PCC 6803 wild type (WT) and mutant strains affected with inactivated DNA methyltransferase genes were analyzed by LC-HDMSE. The mutation of the DNA methyltransferase gene sll0729 encoding M.Ssp6803II initially led to a strong phenoty...
ORGANISM(S): Synechocystis sp. (strain PCC 6803 / Kazusa) 
2019-06-26 | PXD012751 | Pride
Dna methyltransferase Hdmse Label-free quantification Suppressor mutants Synechocystis sp. pcc 6803 
ProteomicsLabel-free quantitative proteomic analysis of DNA methyltransferase mutants of the cyanobacterium Synechocystis sp. PCC 6803 – Analysis of the membrane-enriched fraction
Total protein and membrane-enriched fractions of Synechocystis sp. PCC 6803 wild type (WT) and mutant strains affected with inactivated DNA methyltransferase genes were analyzed by LC-HDMSE. The mutation of the DNA methyltransferase gene sll0729 encoding M.Ssp6803II initially led to a strong phenoty...
ORGANISM(S): Synechocystis sp. (strain PCC 6803 / Kazusa) 
2019-06-26 | PXD012698 | Pride
Dna methyltransferase Hdmse Label-free quantification Suppressor mutants Synechocystis sp. pcc 6803 
ProteomicsMemory-enhancing effects of Origanum majorana essential oil in an Alzheimer's amyloid beta1-42 rat model: a molecular and behavioral study
Origanum L. (Lamiaceae) is an important genus of medicinal and aromatic plants used in the traditional medicine since ancient times as culinary herbs and remedies. The effects of Origanum majorana-based aromatherapy was investigated in an Alzheimer's amyloid beta1-42 rat model. Protein extracts from...
ORGANISM(S): Rattus norvegicus (Rat) 
2020-09-29 | PXD021082 | Pride
Alzheimer disease Rat Amyloid beta1-42 Brain tissue Origanum majorana 
ProteomicsAnalysis of proteins in supernatant and precipitate fractions of human bronchial smooth muscle cells by 1D SDS-PAGE and quantitative LC-MS/MS
Supernatant and precipitate protein fractions of human bronchial smooth muscle cells (HBSMC) after sonication and centrifugation were analyzed using 1D SDS-PAGE. The gel lanes were cube-cut into thirty-three 1.1 mm x 1.1 mm x 1 mm (gel thickness) squares and each gel piece was subjected to in-gel d...
ORGANISM(S): Homo sapiens (Human) 
2019-01-03 | PXD010036 | Pride
Precipitation fraction 1d sds-page Supernatant fraction Bronchial smooth muscle cells Quantitative lc-ms/ms 
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