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Tbrucei_Starve_Synch_MA
ORGANISM(S): Trypanosoma brucei 
Procyclic trypanosomes (strain 427 lister) were grown in culture under standard conditions at 27ºC in SDM79 medium with 10% foetal bovine serume (Brun and Schnenberger, 1979), in a gazed incubator (5% CO2). Logarithmically growing procyclic cells (at about 5*10^6 cells/ml, at 27°C) were added to o...
ORGANISM(S): Trypanosoma brucei 
Induction of the expression of the DHH1 wild type from pLEW100 for 24 hours (M-1 24 hours Tetracycline). Non-induced cells were used as control.
ORGANISM(S): Trypanosoma brucei 
Induction of the expression of the DHH1 DEAD:DQAD mutant from pLEW100 for 24 hours (thus, M-1 24 hours
Tetracycline). Uninduced cells were used as control.
ORGANISM(S): Trypanosoma brucei 
Cy3 and Cy5 direct labelled RNA from Bloodstream MiTat1.1 trypanosomes and Procyclic 427 Lister were hybridized onto JCVI Trypanosoma brucei oligoarrays (version2). Procyclic RNA were used as control for data analysis.
ORGANISM(S): Trypanosoma brucei 
We analysed differentiation of the EATRO1125 strain of Trypanosoma brucei brucei, which was first isolated in 1966 from a bushbuck (Tragelaphus scriptus) in Uganda (origin stated (Bouteillea, 1995) without an original reference.
To analyse gene expression, we isolated at least 3 x 10e8 trypanosom...
ORGANISM(S): Trypanosoma brucei 
In this study we investigate the function of RBP10, and present evidence that the presence of RBP10 is sufficient to induce the expression of mRNAs involved in bloodstream-form energy metabolism. for BS RBP10 RNAi Samples: total of 6 slides, including 3 biological replicates and dyeswap for RBP10 ov...
ORGANISM(S): Trypanosoma brucei 
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