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MetabolomicsCell type-specific proteomics uncovers a RAF15-SnRK2.6/OST1 kinase cascade in guard cells

Multicellular organisms such as plants contain various cell types with specialized functions. Analyzing the characteristics of each cell type reveals specific cell functions and enhances our understanding of organization and function at the organismal level. Guard cells (GCs) are specialized epid...

2024-09-03 | MTBLS3997 | MetaboLights
ProteomicsInteractome dynamics of RAF1-BRAF kinase monomers and dimers
RAF kinases play major roles in cancer. BRAFV600E mutants drive ~6% of human cancers. Potent kinase inhibitors exist but show variable effects in different cancer types, sometimes even inducing paradoxical RAF kinase activation. Both paradoxical activation and drug resistance are frequently due to e...
ORGANISM(S): Homo sapiens (Human) 
2023-02-27 | PXD036792 | Pride
Raf1 Braf Dimerization Raf kinase Interactome 
ProteomicsThe analysis of human RAF1 proxisome
RAF1 is a crucial element of the MAPK/ERK signaling pathway. TurboID, an efficient promiscuous biotin ligase, enables the biotinylation of proteins in the vicinity, thus facilitating the study of complex cellular processes. In this study, we utilized TurboID fused to RAF1WT, RAF1K375M (kinase inacti...
ORGANISM(S): Homo sapiens (Human) 
2025-04-08 | PXD053389 | Pride
Turboid Raf1 kinase Proximity dependent labelling 
ProteomicsRAF1 scaffold integrity shapes chemogenetic degradation outcomes in KRAS-driven lung cancer
Scaffold integrity is essential for the biological activity of proteins that function through protein-protein interactions rather than catalytic output. RAF1 exemplifies this duality: although it is a bona fide kinase and a core component of the MAPK cascade, its tumor-promoting role is largely kina...
ORGANISM(S): Mus musculus (Mouse) 
2026-09-17 | PXD079461 | Pride
Scaffolding functions Lung adenocarcinoma Apoptosis Raf1 
TranscriptomicsEndothelial Raf1 activation regulates arterial morphogenesis
Analyze effect of Raf1 S259A on gene expression in HUVEC Total RNA obtained from HUVEC infected with empty lentiviral vector pLVX-IRES-puro (control) or that expressing human RAF1 WT (WT) or RAF1 S259A (S259A).
ORGANISM(S): Homo sapiens 
2012-12-18 | E-GEOD-42964 | biostudies-arrayexpress
UnknownInteractome dynamics of RAF1-BRAF kinase monomers and dimers.
Not available
S-EPMC10097620 | biostudies-literature
TranscriptomicsTime-course expression data from HEK293∆RAF1:ER cells stimulated with 4OHT, U0126, CYHX, ActD, EGF, FGF, or IGF
We integrate experimental data and mathematical modelling to unveil how ERK signal duration is relayed to mRNA dynamics. HEK293 cells were transfected with an inducible form of RAF (∆RAF1:ER) and induced with tamoxifen (4OHT) or stimulated with different growth factors (EGF, FGF, iGF). Effects of ...
ORGANISM(S): Homo sapiens 
2017-04-28 | E-GEOD-72919 | biostudies-arrayexpress
TranscriptomicsRaf/MEK signatures in zebrafish liver cells
An inducible oncogenic cell line ZFL-?Raf1-ER was established, in which oncogenic human Raf-1(?Raf1) can be activated in zebrafish liver cells by administration of 4-hydroxytamoxifen (4HT). Transcriptional profilling of the ZFL-?Raf1-ER cells with and without administration of 4HT and/or the MEK inh...
ORGANISM(S): Danio rerio 
2011-07-07 | E-GEOD-29308 | biostudies-arrayexpress
ProteomicsIdentification of Mitogen-Activated Protein Kinase Phosphatase-1 (MKP-1) protein partners using Tandem Affinity Purification and Mass Spectrometry
According to the World Health Organization Report, depressive disorders affect about 10% of the population. The molecular mechanism of the pathogenesis of depression is still not well understood. The new findings point to phosphatases as potential targets for effective depression therapy. The aim of...
ORGANISM(S): Rattus Norvegicus (ncbitaxon:10116) 
2023-01-19 | MSV000091093 | MassIVE
UnknownPTEN signaling through RAF1 proto-oncogene serine/threonine kinase (RAF1)/ERK in the epididymis is essential for male fertility.
Not available
S-EPMC4284526 | biostudies-literature
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