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TranscriptomicsAdvancing Trypanosoma brucei genome annotation through ribosome profiling and spliced leader mapping
Since the initial publication of the trypanosomatid genomes, curation has been ongoing. Here we apply the technique of ribosome profiling to Trypanosoma brucei, identifying 223 new coding regions by virtue of ribosome occupancy in the corresponding transcripts. A small number of these putative genes...
ORGANISM(S): Trypanosoma brucei 
2015-12-22 | E-GEOD-72463 | biostudies-arrayexpress
TranscriptomicsGlucosylated hydroxymethyluracil (DNA base J) prevents transcriptional read-through in Leishmania
Some T's in nuclear DNA of trypanosomes and Leishmania are hydroxylated and glucosylated to yield base J (?-D-glucosyl-hydroxymethyluracil). In Leishmania about 99% of J is located in telomeric repeats. We show here that most of the remaining J is located at chromosome-internal RNA Polymerase II ter...
ORGANISM(S): Leishmania major 
2011-10-15 | E-GEOD-32976 | biostudies-arrayexpress
TranscriptomicsMetabolic Reprogramming During Purine Stress in the Protozoan Pathogen Leishmania donovani
The ability of Leishmania to survive in their insect or mammalian host is dependent upon an ability to sense and adapt to changes in the microenvironment. However, little is known about the molecular mechanisms underlying the parasite response to environmental changes, such as nutrient availability....
ORGANISM(S): Leishmania donovani 
2014-02-01 | E-GEOD-48394 | biostudies-arrayexpress
TranscriptomicsKinetoplastid-specific histone variant functions are conserved in Leishmania major
Protein-coding genes in kinetoplastid protists are transcribed from polycistronic arrays, yielding RNA precursors which are processed to form mature transcripts bearing a 5M-bM-^@M-^Y spliced leader (SL) and 3M-bM-^@M-^Y poly(A) tract. Regions of transcription initiation and termination lack known e...
ORGANISM(S): Leishmania major 
2013-10-26 | E-GEOD-51737 | biostudies-arrayexpress
TranscriptomicsStage-specific regulation of translation revealed by ribosome profiling of Trypanosoma brucei
Trypanosoma brucei subspecies infect humans and animals in sub-Saharan Africa. This early diverging eukaryote shows many novel features in basic biological processes, including the use of polycistronic transcription to generate all protein-coding mRNAs. Therefore we hypothesized that translational...
ORGANISM(S): Trypanosoma brucei 
2014-05-24 | E-GEOD-57336 | biostudies-arrayexpress
TranscriptomicsAn arginine sensing pathway in Leishmania induces a Mitogen-Activated Protein Kinase 2-mediated response during macrophage invasion
Protozoa of the genus Leishmania are the causative agents of leishmaniasis in humans. These parasites cycle between promastigotes in the sand fly mid-gut and amastigotes in phagolysosome of mammalian macrophages. During infection, host up-regulate nitric oxide synthase and parasite induce host argin...
ORGANISM(S): Leishmania donovani 
2016-08-01 | E-GEOD-71572 | biostudies-arrayexpress
TranscriptomicsExamining the genetic basis for visceral leishmaniasis disease: Comparison of distinct Leishmania donovani isolates that cause cutaneous and visceral leishmaniasis in Sri Lanka
Among the most central questions in Leishmania research is why some species remain in the skin dermis at the site of infection by the sand fly vector whereas other species migrate to visceral organs where they cause fatal visceral leishmaniasis. Although L. donovani is the species typically responsi...
ORGANISM(S): Leishmania donovani 
2013-12-31 | E-GEOD-48475 | biostudies-arrayexpress
TranscriptomicsMathematical modeling of Trypanosoma brucei transcription
Trypanosome RNA polymerase II transcription is polycistronic, individual mRNAs being excised by trans splicing and polyadenylation. In this study, we refined the previously published mathematical model for bloodstream form parasites and extended it to the procyclic form. We used the model, together ...
ORGANISM(S): Trypanosoma brucei 
2016-03-16 | E-GEOD-79208 | biostudies-arrayexpress
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