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ProteomicsThe value of subcellular fractionation for protein identification in a B-cell line (Ramos)
Integrated in the Chromosomic-centric Human Proteome Project (C-HPP), a lymphocytic B-cell line (Ramos) was selected as a model of B-cells. We performed a subfractionation strategy (in triplicate) for the extraction of proteins from 4 different fractions (cytoplasm, CYT; organella, ORG; membrane, MB...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-07-20 | MSV000079936 | MassIVE
ProteomicsChr16-HPP. Shotgun Analysis improvement. JPR HPP Special issue 2013. Ramos Cell line.
The Chromosome 16 Consortium is integrated in the Human Proteome Project that aims to develop an entire map of the proteins encoded by the human genome following a gene-centric strategy (C-HPP) to make progress in the understanding of human biology in health and disease (B/D-HPP). To do this study f...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-06-12 | MSV000083967 | MassIVE
TranscriptomicsTranscriptional profiling of Ramos germinal center B cells
Transcriptional profiling of Ramos germinal center B cells, comparing untreated cells to cells treated with etoposide, and untreated cells to cells treated with anti-IgM. Both treatments, engagement of the B-cell receptor with anti-IgM and induction of DNA double-strand breaks with etoposide, resul...
ORGANISM(S): Homo sapiens 
2012-01-01 | E-GEOD-23169 | biostudies-arrayexpress
TranscriptomicsChIP-on-chip experiment from Ramos cells to analyze genome-wide CRTC2 binding sites in germinal center B cells
ChIP-on-chip experiment from Ramos cells to analyze genome-wide CRTC2 binding sites in germinal center B cells. The goal was to combine information from our ChIP-on-chip and expression array analyses to compile a list of CRTC2 target genes. CRTC2 was immunoprecipitated from sheared chromatin isolat...
ORGANISM(S): Homo sapiens 
2012-01-01 | E-GEOD-23170 | biostudies-arrayexpress
ProteomicsThe value of subcellular fractionation for protein identification in a B-cell line (Ramos)
Integrated in the Chromosomic-centric Human Proteome Project (C-HPP), a lymphocytic B-cell line (Ramos) was selected as a model of B-cells. We performed a subfractionation strategy (in triplicate) for the extraction of proteins from 4 different fractions (cytoplasm, CYT; organella, ORG; membrane, MB...
ORGANISM(S): Homo sapiens (Human) 
2015-08-04 | PXD001933 | Pride
Cytoplasm B-cell Human Organella Nucleus Orbitrap Ramos Membrane 
GenomicsEffect of hypoxia and treatment with cyclosporine A on chemotaxis of RAMOS B cells
Effect of hypoxia and treatment with cyclosporine A on chemotaxis of RAMOS B cells
PRJNA877646 | ENA
TranscriptomicsCRTC2 couples genotoxic stress and plasma cell differentiation in the germinal center
This SuperSeries is composed of the following subset Series: GSE23169: Transcriptional profiling of Ramos germinal center B cells GSE23170: ChIP-on-chip experiment from Ramos cells to analyze genome-wide CRTC2 binding sites in germinal center B cells Refer to individual Series
ORGANISM(S): Homo sapiens 
2012-01-01 | E-GEOD-23171 | biostudies-arrayexpress
TranscriptomicsDelta4-BAFF an new transcription factor
The purpose of the study was to identify downstream gene targets regulated by a new alternative splice of BAFF (B-Cell Activating factor belonging to the TNF Family) that we called Delta4-BAFF (because it characterized by an alternative splice of exon 4). To do this we used human burkitt's lymphoma ...
ORGANISM(S): Homo sapiens 
2012-09-28 | E-MEXP-3595 | biostudies-arrayexpress
TranscriptomicsExpression profile of MCF7, CCD18 and Ramos human cell lines
To uncover the chromosome 16 associated proteome and to take advantage of the generated knowledge to make progress in human biology in health and disease, a consortium of 15 groups was organized in four working groups: SRM and protein sequencing, antibody and peptide standard, clinical healthcare an...
ORGANISM(S): Homo sapiens 
2013-05-31 | E-GEOD-40168 | biostudies-arrayexpress
ProteomicsChr16-HPP. Shotgun Analysis improvement. JPR HPP Special issue 2013. Jurkat Cell line.
The Chromosome 16 Consortium is integrated in the Human Proteome Project that aims to develop an entire map of the proteins encoded by the human genome following a gene-centric strategy (C-HPP) to make progress in the understanding of human biology in health and disease (B/D-HPP). To do this study f...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-06-12 | MSV000083966 | MassIVE
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