To identify the candidate that directly stabilizes G3BP1 mRNA, the enriched proteins pulled down by G3BP1 probes and anti-RAN antibody were subjected to mass spectrometry (MS) analysis
Fragile X-associated tremor/ataxia syndrome (FXTAS) is a late-onset neurodegenerative disorder caused by transcribed CGG repeat expansions in the 5′ untranslated region of FMR1. Both CGG repeat RNA and repeat-associated non-AUG (RAN) translation of the polyglycine-containing protein FMRpolyG are imp...
Noncoding expansions of a hexanucleotide repeat (GGGGCC) in the C9orf72 gene are the most common cause of familial amyotrophic lateral sclerosis and frontotemporal dementia. Here we report transgenic mice carrying a bacterial artificial chromosome (BAC) containing the full human C9orf72 gene with ei...
Despite untargeted LC-MS/MS data being a powerful approach for large-scale metabolomics analysis, a significant challenge in the field lies in the reproducible and efficient analysis of such data, in particular. The power of R-based analysis workflows lies in their high customizability and adapta...
During mitotic exit, thousands of nuclear pore complexes (NPCs) assemble concomitant with the nuclear envelope to build a transport-competent nucleus. Here, we show that Nup50 plays a crucial role in NPC assembly independent of its well-established function in nuclear transport. RNAi-mediated downre...
