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Cell culture: H9 hESCs were grown using feeder-free conditions for 7 days after passage then either RNA was harvested or embryoid bodies were prepared, cultured in suspension for 4 days, plated on gelatin-coated plates, and cultured for an additional 9 days in embryoid body medium. Detailed protocol...
ORGANISM(S): Homo sapiens 
We used microarrays to detail the global programme of gene expression underlying the disease progression in the mutant mice compared to their control littermates. We collected liver tissue from 1-, 3-, 5-, 7-, 9-, and 11-week-old Npc1+/+ and Npc1-/- mice (N=4), and some additional controls.
ORGANISM(S): Mus musculus 
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